[2] Collection and Storage of Land Plant Samples for Macromolecular Comparisons

Kenneth J. Sytsma, Thomas J. Givnish, James F. Smith, William J. Hahn

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

This chapter describes three practical issues encountered in large-scale macromolecular systematic studies of land plants: (1) obtaining the plant tissue (including sources, tissues, collection, preservation, permits, and vouchers); (2) transport of the plant tissue; and (3) storage of plant tissue or macromolecules. Sources of land plant tissue include field collections. Field collections represent the most important source for land plant tissue; additional advantages include the ability to collect within and among populations, to obtain rare or poorly collected plant species, and to reduce the time prior to DNA or isozyme extraction. An alternative source of field-collected material is through procurement by local botanists who are either specialists for the group under study or knowledgeable about specific collection sites. Collection of land plant tissues is explained in the chapter.

Original languageEnglish
Pages (from-to)23-37
Number of pages15
JournalMethods in Enzymology
Volume224
Issue numberC
DOIs
StatePublished - 1 Jan 1993

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