A heat-shock-inducible eukaryotic expression vecto

Clifford W. Schweinfest; Cheryl L. Jorcyk; Fujiwara Shigeyoshi; Takis S. Papas

doi:10.1016/0378-1119(88)90093-5

A heat-shock-inducible eukaryotic expression vecto

Clifford W. Schweinfest
, Cheryl L. Jorcyk
, Fujiwara Shigeyoshi
, Takis S. Papas

Biological Sciences

National Institutes of Health

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We describe a construct, pHS/Cla, containing the Drosophila melanogaster hsp70 promoter which serves as an inducible expression vector in mammalian cells. The construct was made in the plasmid pAT 153, a derivative of pBR322. In transient transfections of human H9 T-cells, the transactivator of transcription protein of the human AIDS virus HIV-1 was functionally expressed in response to heat shock. The promoter is very tightly regulated in that no expression can be detected at 37°C. Expression is highly induced upon heat shock and lasts at least 4 h. The construct contains a unique ClaI site for cloning and expressing potentially any gene.

Original language	English
Pages (from-to)	207-210
Number of pages	4
Journal	Gene
Volume	71
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(88)90093-5
State	Published - 15 Nov 1988

Keywords

AIDS
DNA transfection
HIV-1
hsp70 promoter
pHS/Cla
Recombinant DNA
transactivator of transcription

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10.1016/0378-1119(88)90093-5

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title = "A heat-shock-inducible eukaryotic expression vecto",

abstract = "We describe a construct, pHS/Cla, containing the Drosophila melanogaster hsp70 promoter which serves as an inducible expression vector in mammalian cells. The construct was made in the plasmid pAT 153, a derivative of pBR322. In transient transfections of human H9 T-cells, the transactivator of transcription protein of the human AIDS virus HIV-1 was functionally expressed in response to heat shock. The promoter is very tightly regulated in that no expression can be detected at 37°C. Expression is highly induced upon heat shock and lasts at least 4 h. The construct contains a unique ClaI site for cloning and expressing potentially any gene.",

keywords = "AIDS, DNA transfection, HIV-1, hsp70 promoter, pHS/Cla, Recombinant DNA, transactivator of transcription",

author = "Schweinfest, \{Clifford W.\} and Jorcyk, \{Cheryl L.\} and Fujiwara Shigeyoshi and Papas, \{Takis S.\}",

year = "1988",

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doi = "10.1016/0378-1119(88)90093-5",

language = "English",

volume = "71",

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T1 - A heat-shock-inducible eukaryotic expression vecto

AU - Schweinfest, Clifford W.

AU - Jorcyk, Cheryl L.

AU - Shigeyoshi, Fujiwara

AU - Papas, Takis S.

PY - 1988/11/15

Y1 - 1988/11/15

N2 - We describe a construct, pHS/Cla, containing the Drosophila melanogaster hsp70 promoter which serves as an inducible expression vector in mammalian cells. The construct was made in the plasmid pAT 153, a derivative of pBR322. In transient transfections of human H9 T-cells, the transactivator of transcription protein of the human AIDS virus HIV-1 was functionally expressed in response to heat shock. The promoter is very tightly regulated in that no expression can be detected at 37°C. Expression is highly induced upon heat shock and lasts at least 4 h. The construct contains a unique ClaI site for cloning and expressing potentially any gene.

AB - We describe a construct, pHS/Cla, containing the Drosophila melanogaster hsp70 promoter which serves as an inducible expression vector in mammalian cells. The construct was made in the plasmid pAT 153, a derivative of pBR322. In transient transfections of human H9 T-cells, the transactivator of transcription protein of the human AIDS virus HIV-1 was functionally expressed in response to heat shock. The promoter is very tightly regulated in that no expression can be detected at 37°C. Expression is highly induced upon heat shock and lasts at least 4 h. The construct contains a unique ClaI site for cloning and expressing potentially any gene.

KW - AIDS

KW - DNA transfection

KW - HIV-1

KW - hsp70 promoter

KW - pHS/Cla

KW - Recombinant DNA

KW - transactivator of transcription

UR - https://www.scopus.com/pages/publications/0024272202

U2 - 10.1016/0378-1119(88)90093-5

DO - 10.1016/0378-1119(88)90093-5

M3 - Article

C2 - 2850976

AN - SCOPUS:0024272202

SN - 0378-1119

VL - 71

SP - 207

EP - 210

JO - Gene

JF - Gene

IS - 1

ER -

A heat-shock-inducible eukaryotic expression vecto

Abstract

Keywords

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