A magnetic manipulator for studying local rheology and micromechanical properties of biological systems

François Amblard; Bernard Yurke; Andrew Pargellis; Stanislas Leibler

doi:10.1063/1.1146816

A magnetic manipulator for studying local rheology and micromechanical properties of biological systems

François Amblard, Bernard Yurke, Andrew Pargellis, Stanislas Leibler

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164 Scopus citations

Abstract

A magnetic micromanipulator capable of generating two-dimensional translational and rotational motions on a microscope stage is described. With 3 μm-diam paramagnetic beads, forces in the piconewton range and torques on the order of 10^-14 N m are obtained and can be modulated in time at moderate frequencies (<5 Hz). Typical magnetic fields between 0.1 and 0.2 T, and gradients between 10 and 20 Tm^-1 are created by four independent feedback-controlled electromagnets. Video microscopy and computerized image analysis are used to locate the beads on each image with a resolution of 0.1 pixel (20 nm). The device is primarily designed to study, at a microscopic scale, the local mechanical properties of biological polymers such as actin in solution, and of cell cytoplasm. Possible applications include the in situ manipulation of intracellular organelles.

Original language	English
Pages (from-to)	818-827
Number of pages	10
Journal	Review of Scientific Instruments
Volume	67
Issue number	3
DOIs	https://doi.org/10.1063/1.1146816
State	Published - Mar 1996
Externally published	Yes

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10.1063/1.1146816

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title = "A magnetic manipulator for studying local rheology and micromechanical properties of biological systems",

abstract = "A magnetic micromanipulator capable of generating two-dimensional translational and rotational motions on a microscope stage is described. With 3 μm-diam paramagnetic beads, forces in the piconewton range and torques on the order of 10-14 N m are obtained and can be modulated in time at moderate frequencies (<5 Hz). Typical magnetic fields between 0.1 and 0.2 T, and gradients between 10 and 20 Tm-1 are created by four independent feedback-controlled electromagnets. Video microscopy and computerized image analysis are used to locate the beads on each image with a resolution of 0.1 pixel (20 nm). The device is primarily designed to study, at a microscopic scale, the local mechanical properties of biological polymers such as actin in solution, and of cell cytoplasm. Possible applications include the in situ manipulation of intracellular organelles.",

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AU - Yurke, Bernard

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N2 - A magnetic micromanipulator capable of generating two-dimensional translational and rotational motions on a microscope stage is described. With 3 μm-diam paramagnetic beads, forces in the piconewton range and torques on the order of 10-14 N m are obtained and can be modulated in time at moderate frequencies (<5 Hz). Typical magnetic fields between 0.1 and 0.2 T, and gradients between 10 and 20 Tm-1 are created by four independent feedback-controlled electromagnets. Video microscopy and computerized image analysis are used to locate the beads on each image with a resolution of 0.1 pixel (20 nm). The device is primarily designed to study, at a microscopic scale, the local mechanical properties of biological polymers such as actin in solution, and of cell cytoplasm. Possible applications include the in situ manipulation of intracellular organelles.

AB - A magnetic micromanipulator capable of generating two-dimensional translational and rotational motions on a microscope stage is described. With 3 μm-diam paramagnetic beads, forces in the piconewton range and torques on the order of 10-14 N m are obtained and can be modulated in time at moderate frequencies (<5 Hz). Typical magnetic fields between 0.1 and 0.2 T, and gradients between 10 and 20 Tm-1 are created by four independent feedback-controlled electromagnets. Video microscopy and computerized image analysis are used to locate the beads on each image with a resolution of 0.1 pixel (20 nm). The device is primarily designed to study, at a microscopic scale, the local mechanical properties of biological polymers such as actin in solution, and of cell cytoplasm. Possible applications include the in situ manipulation of intracellular organelles.

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A magnetic manipulator for studying local rheology and micromechanical properties of biological systems

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