An electrophoretic mobility shift assay for methionine sulfoxide in proteins

Christopher C. Saunders, Wesley E. Stites

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Study of the posttranslational modification of methionine to its sulfoxide has been receiving increasing attention because of its implication in regulation of protein activity, but techniques for the detection of this modification remain limited. In particular, there has been no method to detect the oxidation of methionine on polyacrylamide gels. Here we demonstrate that alkylation of methionine introduces a charge change that shifts the mobility of the protein on an acidic gel relative to the alkylation-resistant sulfoxide form.

Original languageEnglish
Pages (from-to)767-769
Number of pages3
JournalAnalytical Biochemistry
Volume421
Issue number2
DOIs
StatePublished - 15 Feb 2012

Keywords

  • Acid PAGE gel
  • Methionine alkylation
  • Methionine oxidation
  • Methionine sulfonium

Fingerprint

Dive into the research topics of 'An electrophoretic mobility shift assay for methionine sulfoxide in proteins'. Together they form a unique fingerprint.

Cite this