TY - JOUR
T1 - An electrophoretic mobility shift assay for methionine sulfoxide in proteins
AU - Saunders, Christopher C.
AU - Stites, Wesley E.
PY - 2012/2/15
Y1 - 2012/2/15
N2 - Study of the posttranslational modification of methionine to its sulfoxide has been receiving increasing attention because of its implication in regulation of protein activity, but techniques for the detection of this modification remain limited. In particular, there has been no method to detect the oxidation of methionine on polyacrylamide gels. Here we demonstrate that alkylation of methionine introduces a charge change that shifts the mobility of the protein on an acidic gel relative to the alkylation-resistant sulfoxide form.
AB - Study of the posttranslational modification of methionine to its sulfoxide has been receiving increasing attention because of its implication in regulation of protein activity, but techniques for the detection of this modification remain limited. In particular, there has been no method to detect the oxidation of methionine on polyacrylamide gels. Here we demonstrate that alkylation of methionine introduces a charge change that shifts the mobility of the protein on an acidic gel relative to the alkylation-resistant sulfoxide form.
KW - Acid PAGE gel
KW - Methionine alkylation
KW - Methionine oxidation
KW - Methionine sulfonium
UR - http://www.scopus.com/inward/record.url?scp=84859722209&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2011.12.021
DO - 10.1016/j.ab.2011.12.021
M3 - Article
C2 - 22230286
AN - SCOPUS:84859722209
SN - 0003-2697
VL - 421
SP - 767
EP - 769
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -