TY - JOUR
T1 - Backbone 1H, 13C and 15N assignments of the apo-acyl carrier protein (ACP1) of Pseudomonas aeruginosa
AU - Rizzo, Madison
AU - Baggs, Eric
AU - Chowdhury, Abu Sayeed
AU - Nagarajan, Rajesh
AU - Warner, Lisa Rose
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2023/12
Y1 - 2023/12
N2 - The N-acyl-L-homoserine lactone (AHL) quorum sensing regulates virulence in the opportunistic pathogen, Pseudomonas aeruginosa. The LasI and RhlI AHL synthases use acyl carrier protein substrates to synthesize, respectively, the 3-oxododecanoyl-L-homoserine lactone (3-oxoC12-HSL) and butyryl-L-homoserine lactone (C4-HSL) QS signals for this bacterium. Although P. aeruginosa genome contains three open reading frames to encode three acyl carrier proteins, namely the ACP1, ACP2 and ACP3, microarray and gene replacement studies show that only the ACP1 carrier protein is under quorum sensing regulation. In this study, we isotopically enriched one of the acyl carrier proteins, ACP1 from P. aeruginosa and describe the backbone resonance assignments for this protein to delineate the structural and molecular basis of ACP1 recognition in P. aeruginosa AHL quorum sensing signal synthesis.
AB - The N-acyl-L-homoserine lactone (AHL) quorum sensing regulates virulence in the opportunistic pathogen, Pseudomonas aeruginosa. The LasI and RhlI AHL synthases use acyl carrier protein substrates to synthesize, respectively, the 3-oxododecanoyl-L-homoserine lactone (3-oxoC12-HSL) and butyryl-L-homoserine lactone (C4-HSL) QS signals for this bacterium. Although P. aeruginosa genome contains three open reading frames to encode three acyl carrier proteins, namely the ACP1, ACP2 and ACP3, microarray and gene replacement studies show that only the ACP1 carrier protein is under quorum sensing regulation. In this study, we isotopically enriched one of the acyl carrier proteins, ACP1 from P. aeruginosa and describe the backbone resonance assignments for this protein to delineate the structural and molecular basis of ACP1 recognition in P. aeruginosa AHL quorum sensing signal synthesis.
KW - Acyl carrier protein (ACP)
KW - N-acyl-L-homoserine lactone synthases
KW - Nuclear magnetic resonance
KW - Pseudomonas aeruginosa
KW - Quorum sensing
KW - RhlI
UR - http://www.scopus.com/inward/record.url?scp=85164135542&partnerID=8YFLogxK
U2 - 10.1007/s12104-023-10138-2
DO - 10.1007/s12104-023-10138-2
M3 - Article
C2 - 37421542
AN - SCOPUS:85164135542
SN - 1874-2718
VL - 17
SP - 183
EP - 188
JO - Biomolecular NMR Assignments
JF - Biomolecular NMR Assignments
IS - 2
ER -