Backbone 1H, 13C and 15N assignments of the apo-acyl carrier protein (ACP1) of Pseudomonas aeruginosa

Madison Rizzo, Eric Baggs, Abu Sayeed Chowdhury, Rajesh Nagarajan, Lisa Rose Warner

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The N-acyl-L-homoserine lactone (AHL) quorum sensing regulates virulence in the opportunistic pathogen, Pseudomonas aeruginosa. The LasI and RhlI AHL synthases use acyl carrier protein substrates to synthesize, respectively, the 3-oxododecanoyl-L-homoserine lactone (3-oxoC12-HSL) and butyryl-L-homoserine lactone (C4-HSL) QS signals for this bacterium. Although P. aeruginosa genome contains three open reading frames to encode three acyl carrier proteins, namely the ACP1, ACP2 and ACP3, microarray and gene replacement studies show that only the ACP1 carrier protein is under quorum sensing regulation. In this study, we isotopically enriched one of the acyl carrier proteins, ACP1 from P. aeruginosa and describe the backbone resonance assignments for this protein to delineate the structural and molecular basis of ACP1 recognition in P. aeruginosa AHL quorum sensing signal synthesis.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalBiomolecular NMR Assignments
Volume17
Issue number2
DOIs
StatePublished - Dec 2023

Keywords

  • Acyl carrier protein (ACP)
  • N-acyl-L-homoserine lactone synthases
  • Nuclear magnetic resonance
  • Pseudomonas aeruginosa
  • Quorum sensing
  • RhlI

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