Bacteria of the Sagebrush Phyllosphere...or Is It?

Chadwick DeFehr, Jacob Heil, Leonora Bittleston

Research output: Contribution to conferencePoster

Abstract

While previous work has made successful strides in characterizing the fungi that live on sagebrush ( Artemisia tridentate ) leaves, characterizing the bacteria that call these leaves home presents different challenges. We used a wash in water alone to suspend the phyllosphere inhabitants and the Oxford Nanopore minION DNA sequencer to sequence their DNA. Many cyanobacteria were described using the epi2me software as the primary component of phyllosphere bacteria. This led to different methods of investigating the taxonomy of our sequences and the likelihoods that they are nearly all from chloroplasts or mitochondria, despite their assignment as bacteria in the Epi2Me workflow.

Original languageAmerican English
StatePublished - 1 Jul 2023
EventIdaho Conference on Undergraduate Research 2023 - Boise State University, Boise, United States
Duration: 1 Jul 2023 → …
https://scholarworks.boisestate.edu/icur/2023/

Conference

ConferenceIdaho Conference on Undergraduate Research 2023
Abbreviated titleICUR 2023
Country/TerritoryUnited States
CityBoise
Period1/07/23 → …
Internet address

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