TY - JOUR
T1 - Bone marrow fat accumulation accelerated by high fat diet is suppressed by exercise
AU - Styner, Maya
AU - Thompson, William R.
AU - Galior, Kornelia
AU - Uzer, Gunes
AU - Wu, Xin
AU - Kadari, Sanjay
AU - Case, Natasha
AU - Xie, Zhihui
AU - Sen, Buer
AU - Romaine, Andrew
AU - Pagnotti, Gabriel M.
AU - Rubin, Clinton T.
AU - Styner, Martin A.
AU - Horowitz, Mark C.
AU - Rubin, Janet
PY - 2014/7
Y1 - 2014/7
N2 - Marrow adipose tissue (MAT), associated with skeletal fragility and hematologic insufficiency, remains poorly understood and difficult to quantify. We tested the response of MAT to high fat diet (HFD) and exercise using a novel volumetric analysis, and compared it to measures of bone quantity. We hypothesized that HFD would increase MAT and diminish bone quantity, while exercise would slow MAT acquisition and promote bone formation. Eight week-old female C57BL/6 mice were fed a regular (RD) or HFD, and exercise groups were provided voluntary access to running wheels (RD-E, HFD-E). Femoral MAT was assessed by μCT (lipid binder osmium) using a semi-automated approach employing rigid co-alignment, regional bone masks and was normalized for total femoral volume (TV) of the bone compartment. MAT was 2.6-fold higher in HFD relative to RD mice. Exercise suppressed MAT in RD-E mice by more than half compared with RD. Running similarly inhibited MAT acquisition in HFD mice. Exercise significantly increased bone quantity in both diet groups. Thus, HFD caused significant accumulation of MAT; importantly running exercise limited MAT acquisition while promoting bone formation during both diets. That MAT is exquisitely responsive to diet and exercise, and its regulation by exercise appears to be inversely proportional to effects on exercise induced bone formation, is relevant for an aging and sedentary population.
AB - Marrow adipose tissue (MAT), associated with skeletal fragility and hematologic insufficiency, remains poorly understood and difficult to quantify. We tested the response of MAT to high fat diet (HFD) and exercise using a novel volumetric analysis, and compared it to measures of bone quantity. We hypothesized that HFD would increase MAT and diminish bone quantity, while exercise would slow MAT acquisition and promote bone formation. Eight week-old female C57BL/6 mice were fed a regular (RD) or HFD, and exercise groups were provided voluntary access to running wheels (RD-E, HFD-E). Femoral MAT was assessed by μCT (lipid binder osmium) using a semi-automated approach employing rigid co-alignment, regional bone masks and was normalized for total femoral volume (TV) of the bone compartment. MAT was 2.6-fold higher in HFD relative to RD mice. Exercise suppressed MAT in RD-E mice by more than half compared with RD. Running similarly inhibited MAT acquisition in HFD mice. Exercise significantly increased bone quantity in both diet groups. Thus, HFD caused significant accumulation of MAT; importantly running exercise limited MAT acquisition while promoting bone formation during both diets. That MAT is exquisitely responsive to diet and exercise, and its regulation by exercise appears to be inversely proportional to effects on exercise induced bone formation, is relevant for an aging and sedentary population.
KW - Bone micro architecture
KW - Exercise
KW - Lipid
KW - Marrow adipose tissue
KW - Micro-CT
KW - Quantitative image analysis
UR - http://www.scopus.com/inward/record.url?scp=84898855114&partnerID=8YFLogxK
U2 - 10.1016/j.bone.2014.03.044
DO - 10.1016/j.bone.2014.03.044
M3 - Article
C2 - 24709686
AN - SCOPUS:84898855114
SN - 8756-3282
VL - 64
SP - 39
EP - 46
JO - Bone
JF - Bone
ER -