Bystander-Mediated Stimulation of Proteolipid Protein-Specific Regulatory T (Treg) Cells Confers Protection Against Experimental Autoimmune Encephalomyelitis (EAE) via TGF-β

SangMu Jun; Javier Ochoa-Reparaz; Dagmara Zlotkowska; Teri Hoyt; David W. Pascual

doi:10.1016/j.jneuroim.2012.02.003

Bystander-Mediated Stimulation of Proteolipid Protein-Specific Regulatory T (T_reg) Cells Confers Protection Against Experimental Autoimmune Encephalomyelitis (EAE) via TGF-β

SangMu Jun
, Javier Ochoa-Reparaz
, Dagmara Zlotkowska
, Teri Hoyt
, David W. Pascual

Montana State University

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

To assess the potency of regulatory T (T reg ) cells induced against an irrelevant Ag, mice were orally vaccinated with Salmonella expressing Escherichia coli colonization factor antigen I fimbriae. Isolated CD25 + and CD25 − CD4 + T cells were adoptively transferred to naive mice, and T reg cells effectively protected against experimental autoimmune encephalomyelitis (EAE), unlike T reg cells from Salmonella vector-immunized mice. This protection was abrogated upon in vivo neutralization of TGF-β, resulting in elevated IL-17 and loss of IL-4 and IL-10 production. Thus, T reg cells induced to irrelevant Ags offer a novel approach to treat autoimmune diseases independent of auto-Ag.

Original language	American English
Pages (from-to)	39-47
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	245
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2012.02.003
State	Published - Apr 2012
Externally published	Yes

Keywords

Salmonella
TGF-β
Treg cells
experimental autoimmune encephalomyelitis

EGS Disciplines

Biology

Access to Document

10.1016/j.jneuroim.2012.02.003License: Unspecified

Cite this

@article{c402a77cb0744fac958f886ceedfc8fc,

title = "Bystander-Mediated Stimulation of Proteolipid Protein-Specific Regulatory T (Treg) Cells Confers Protection Against Experimental Autoimmune Encephalomyelitis (EAE) via TGF-β",

abstract = " To assess the potency of regulatory T (T reg ) cells induced against an irrelevant Ag, mice were orally vaccinated with Salmonella expressing Escherichia coli colonization factor antigen I fimbriae. Isolated CD25 + and CD25 − CD4 + T cells were adoptively transferred to naive mice, and T reg cells effectively protected against experimental autoimmune encephalomyelitis (EAE), unlike T reg cells from Salmonella vector-immunized mice. This protection was abrogated upon in vivo neutralization of TGF-β, resulting in elevated IL-17 and loss of IL-4 and IL-10 production. Thus, T reg cells induced to irrelevant Ags offer a novel approach to treat autoimmune diseases independent of auto-Ag. ",

keywords = "Salmonella, TGF-β, Treg cells, experimental autoimmune encephalomyelitis",

author = "SangMu Jun and Javier Ochoa-Reparaz and Dagmara Zlotkowska and Teri Hoyt and Pascual, \{David W.\}",

year = "2012",

month = apr,

doi = "10.1016/j.jneuroim.2012.02.003",

language = "American English",

volume = "245",

pages = "39--47",

number = "1-2",

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Bystander-Mediated Stimulation of Proteolipid Protein-Specific Regulatory T (T_reg) Cells Confers Protection Against Experimental Autoimmune Encephalomyelitis (EAE) via TGF-β. / Jun, SangMu; Ochoa-Reparaz, Javier; Zlotkowska, Dagmara et al.
In: Journal of Neuroimmunology, Vol. 245, No. 1-2, 04.2012, p. 39-47.

Research output: Contribution to journal › Article › peer-review

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AU - Pascual, David W.

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AB - To assess the potency of regulatory T (T reg ) cells induced against an irrelevant Ag, mice were orally vaccinated with Salmonella expressing Escherichia coli colonization factor antigen I fimbriae. Isolated CD25 + and CD25 − CD4 + T cells were adoptively transferred to naive mice, and T reg cells effectively protected against experimental autoimmune encephalomyelitis (EAE), unlike T reg cells from Salmonella vector-immunized mice. This protection was abrogated upon in vivo neutralization of TGF-β, resulting in elevated IL-17 and loss of IL-4 and IL-10 production. Thus, T reg cells induced to irrelevant Ags offer a novel approach to treat autoimmune diseases independent of auto-Ag.

KW - Salmonella

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