TY - JOUR
T1 - CD40 is functionally expressed on human breast carcinomas
T2 - Variable inducibility by cytokines and enhancement of Fas-mediated apoptosis
AU - Wingett, Denise G.
AU - Vestal, Robert E.
AU - Forcier, Kristin
AU - Hadjokas, Nicholas
AU - Nielson, Christopher P.
PY - 1998
Y1 - 1998
N2 - The CD40 molecule, a member of the TNF receptor gene family, has been intensively studied with respect to regulation of B cell proliferation and survival. Although CD40 is also expressed on carcinoma cell lines, information concerning the biological function of CD40 on cells of epithelial origin is limited. In this study we detected constitutive CD40 on human breast carcinoma cell lines and an increase in CD40 expression following treatment with cytokines IL-1α and IFN-γ. CD40 ligation was also found to increase MHC II expression in cells pretreated with IFN-γ. In contrast to normal B cells, where CD40 signaling provides a potent survival signal, we observed that CD40 ligation in breast carcinoma cells results in growth inhibition and enhanced susceptibility to Fas-mediated apoptosis. Enhanced apoptosis appears to be attributable, at least in part, to an up-regulation of Fas expression caused by CD40 ligation. These results suggest a potentially important role for CD40 in breast tumor biology.
AB - The CD40 molecule, a member of the TNF receptor gene family, has been intensively studied with respect to regulation of B cell proliferation and survival. Although CD40 is also expressed on carcinoma cell lines, information concerning the biological function of CD40 on cells of epithelial origin is limited. In this study we detected constitutive CD40 on human breast carcinoma cell lines and an increase in CD40 expression following treatment with cytokines IL-1α and IFN-γ. CD40 ligation was also found to increase MHC II expression in cells pretreated with IFN-γ. In contrast to normal B cells, where CD40 signaling provides a potent survival signal, we observed that CD40 ligation in breast carcinoma cells results in growth inhibition and enhanced susceptibility to Fas-mediated apoptosis. Enhanced apoptosis appears to be attributable, at least in part, to an up-regulation of Fas expression caused by CD40 ligation. These results suggest a potentially important role for CD40 in breast tumor biology.
KW - Apoptosis
KW - Breast-neoplasms
KW - Carcinoma
KW - CD40
KW - Fas
KW - IFN-γ
UR - http://www.scopus.com/inward/record.url?scp=0031788449&partnerID=8YFLogxK
U2 - 10.1023/A:1006012607452
DO - 10.1023/A:1006012607452
M3 - Article
C2 - 9802617
AN - SCOPUS:0031788449
SN - 0167-6806
VL - 50
SP - 27
EP - 36
JO - Breast Cancer Research and Treatment
JF - Breast Cancer Research and Treatment
IS - 1
ER -