Characterization of Humic Acid Size Fractions by SEC and MALS

Ray von Wandruszka; Martin Schimpf; Michael Hill; Regginal Engebretson

doi:10.1016/S0146-6380(98)00217-4

Characterization of Humic Acid Size Fractions by SEC and MALS

Ray von Wandruszka
, Martin Schimpf
, Michael Hill
, Regginal Engebretson

Chemistry Department

Boise State University
University of Idaho

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Latahco silt-loam humic acid was separated on a preparatory scale by size exclusion chromatography (SEC) on a gravity-fed Sepharose column. Four fractions from this separation were collected and further analyzed, along with whole humic acid, by high-performance SEC coupled with a multi-angle light scattering (MALS) detector. A detailed characterization of the radius of gyration across the elution profile of each fraction by MALS demonstrated that the preparative column generally separated the humic acid according to differences in molecular size. Furthermore, a comparison of elution profiles obtained on the whole humic acid with UV and refractive index detectors revealed the presence of a non-aromatic low molecular weight component.

Original language	American English
Journal	Organic Geochemistry
DOIs	https://doi.org/10.1016/S0146-6380(98)00217-4
State	Published - 1 Apr 1999

Keywords

HPSEC
Humic acid
multi-angle light scattering
size-exclusion chromatography

EGS Disciplines

Chemistry

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http://dx.doi.org/10.1016/S0146-6380(98)00217-4

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title = "Characterization of Humic Acid Size Fractions by SEC and MALS",

abstract = " Latahco silt-loam humic acid was separated on a preparatory scale by size exclusion chromatography (SEC) on a gravity-fed Sepharose column. Four fractions from this separation were collected and further analyzed, along with whole humic acid, by high-performance SEC coupled with a multi-angle light scattering (MALS) detector. A detailed characterization of the radius of gyration across the elution profile of each fraction by MALS demonstrated that the preparative column generally separated the humic acid according to differences in molecular size. Furthermore, a comparison of elution profiles obtained on the whole humic acid with UV and refractive index detectors revealed the presence of a non-aromatic low molecular weight component.",

keywords = "HPSEC, Humic acid, multi-angle light scattering, size-exclusion chromatography",

author = "Wandruszka, \{Ray von\} and Martin Schimpf and Michael Hill and Regginal Engebretson",

year = "1999",

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doi = "10.1016/S0146-6380(98)00217-4",

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TY - JOUR

T1 - Characterization of Humic Acid Size Fractions by SEC and MALS

AU - Wandruszka, Ray von

AU - Schimpf, Martin

AU - Hill, Michael

AU - Engebretson, Regginal

PY - 1999/4/1

Y1 - 1999/4/1

N2 - Latahco silt-loam humic acid was separated on a preparatory scale by size exclusion chromatography (SEC) on a gravity-fed Sepharose column. Four fractions from this separation were collected and further analyzed, along with whole humic acid, by high-performance SEC coupled with a multi-angle light scattering (MALS) detector. A detailed characterization of the radius of gyration across the elution profile of each fraction by MALS demonstrated that the preparative column generally separated the humic acid according to differences in molecular size. Furthermore, a comparison of elution profiles obtained on the whole humic acid with UV and refractive index detectors revealed the presence of a non-aromatic low molecular weight component.

AB - Latahco silt-loam humic acid was separated on a preparatory scale by size exclusion chromatography (SEC) on a gravity-fed Sepharose column. Four fractions from this separation were collected and further analyzed, along with whole humic acid, by high-performance SEC coupled with a multi-angle light scattering (MALS) detector. A detailed characterization of the radius of gyration across the elution profile of each fraction by MALS demonstrated that the preparative column generally separated the humic acid according to differences in molecular size. Furthermore, a comparison of elution profiles obtained on the whole humic acid with UV and refractive index detectors revealed the presence of a non-aromatic low molecular weight component.

KW - HPSEC

KW - Humic acid

KW - multi-angle light scattering

KW - size-exclusion chromatography

UR - https://scholarworks.boisestate.edu/chem_facpubs/45

UR - http://dx.doi.org/10.1016/S0146-6380(98)00217-4

U2 - 10.1016/S0146-6380(98)00217-4

DO - 10.1016/S0146-6380(98)00217-4

M3 - Article

JO - Organic Geochemistry

JF - Organic Geochemistry

ER -

Characterization of Humic Acid Size Fractions by SEC and MALS

Abstract

Keywords

EGS Disciplines

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