Collection and Storage of Land Plant Samples for Macromolecular Comparisons

Kenneth J. Sytsma, Thomas J. Givnish, James F. Smith, William J. Hahn

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

24 Scopus citations

Abstract

This chapter describes three practical issues encountered in large-scale macromolecular systematic studies of land plants: (1) obtaining the plant tissue (including sources, tissues, collection, preservation, permits, and vouchers); (2) transport of the plant tissue; and (3) storage of plant tissue or macromolecules. Sources of land plant tissue include field collections. Field collections represent the most important source for land plant tissue; additional advantages include the ability to collect within and among populations, to obtain rare or poorly collected plant species, and to reduce the time prior to DNA or isozyme extraction. An alternative source of field-collected material is through procurement by local botanists who are either specialists for the group under study or knowledgeable about specific collection sites. Collection of land plant tissues is explained in the chapter.

Original languageEnglish
Title of host publicationMethods in Enzymology
EditorsElizabeth A. Zimmer, Thomas J. White, Rebecca L. Cann, Allan C. Wilson
PublisherElsevier Inc.
Chapter2
Pages23-37
Number of pages14
Volume224
EditionC
ISBN (Print)978-0-12-182125-8
DOIs
StatePublished - 1993

Publication series

NameMethods in Enzymology
PublisherAcademic Press Inc.
ISSN (Print)0076-6879

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