TY - JOUR
T1 - Comparison of detoxification enzyme mRNAS in woodrats (Neotoma lepida) and laboratory rats
AU - Lamb, John G.
AU - Sorensen, Jennifer S.
AU - Dearing, M. Denise
PY - 2001
Y1 - 2001
N2 - To understand how mammalian herbivores process plant secondary compounds, we examined differences in expression of biotransformation enzyme mRNAs among populations of wild woodrats (Neotoma lepida) and laboratory rats. We compared expression of mRNAs for 10 biotransforming enzymes in five families (CYP, mEH, QOR, GST, and UGT) by using Northern blot analysis. We found significant differences in eight of 10 mRNAs tested. We suggest that the differences in mRNA expression among populations of woodrats and laboratory rats may be due to differences in the secondary compound composition of their diets. Our results provide background for future studies of detoxification strategies in mammalian herbivores that combine pharmacological techniques with an ecological perspective.
AB - To understand how mammalian herbivores process plant secondary compounds, we examined differences in expression of biotransformation enzyme mRNAs among populations of wild woodrats (Neotoma lepida) and laboratory rats. We compared expression of mRNAs for 10 biotransforming enzymes in five families (CYP, mEH, QOR, GST, and UGT) by using Northern blot analysis. We found significant differences in eight of 10 mRNAs tested. We suggest that the differences in mRNA expression among populations of woodrats and laboratory rats may be due to differences in the secondary compound composition of their diets. Our results provide background for future studies of detoxification strategies in mammalian herbivores that combine pharmacological techniques with an ecological perspective.
KW - Biotransforming enzymes
KW - Detoxification
KW - Neotoma woodrats
KW - Plant secondary compounds
KW - Xenobiotics
UR - http://www.scopus.com/inward/record.url?scp=0034952360&partnerID=8YFLogxK
U2 - 10.1023/A:1010366322299
DO - 10.1023/A:1010366322299
M3 - Article
C2 - 11446304
AN - SCOPUS:0034952360
SN - 0098-0331
VL - 27
SP - 845
EP - 857
JO - Journal of Chemical Ecology
JF - Journal of Chemical Ecology
IS - 4
ER -