TY - JOUR
T1 - Controlled Gating of Lysenin Pores
AU - Fologea, Daniel
AU - Krueger, Eric
AU - Lee, Rachel
AU - Naglak, Matt
AU - Mazur, Yuriy
AU - Henry, Ralph
AU - Salamo, Greg
PY - 2010/1
Y1 - 2010/1
N2 - Lysenin forms unitary large conductance pores in artificial bilayer membranes containing sphingomyelin. A population of lysenin pores inserted into such a bilayer membrane exhibited a dynamic negative conductance region, as predicted by a simple two-state model for voltage-gated channels. The recorded I-V curves demonstrated that lysenin pores inserted into the bilayer are uniformly oriented. Additionally, the transition between the two-states was affected by changes in the monovalent ion concentration and pH, pointing towards an electrostatic interaction governing the gating mechanism.
AB - Lysenin forms unitary large conductance pores in artificial bilayer membranes containing sphingomyelin. A population of lysenin pores inserted into such a bilayer membrane exhibited a dynamic negative conductance region, as predicted by a simple two-state model for voltage-gated channels. The recorded I-V curves demonstrated that lysenin pores inserted into the bilayer are uniformly oriented. Additionally, the transition between the two-states was affected by changes in the monovalent ion concentration and pH, pointing towards an electrostatic interaction governing the gating mechanism.
KW - Lysenin
KW - Negative conductance
KW - Voltage gated pores
UR - http://www.scopus.com/inward/record.url?scp=70449704033&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1016/j.bpc.2009.09.014
U2 - 10.1016/j.bpc.2009.09.014
DO - 10.1016/j.bpc.2009.09.014
M3 - Article
C2 - 19854558
AN - SCOPUS:70449704033
SN - 0301-4622
VL - 146
SP - 25
EP - 29
JO - Biophysical Chemistry
JF - Biophysical Chemistry
IS - 1
ER -