Cross-Correlation Continuous Capillary Electrophoresis: Resolution, Processing Time, and Concentration Effects: Resolution, processing time, and concentration effects

Martin Schimpf, Semen N. Semenov

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

An expression is derived that relates the signal-to-noise (S/N) ratio in continuous-mode electrophoresis to system parameters. In this process, the time-based cross-correlation function (TCCF) is computed from signals caused by individual analyte passage through two separate detectors. As a characteristic of the noise, the cross-variance of the TCCF is proposed, so that the S/N ratio is defined as the TCCF divided by the square root of the cross-variance. From the derived expressions, the important parameters for method optimization are identified. The dependence of the S/N ratio on system parameters varies with analyte size due to a contribution from the variance in the flight time, which is affected by particle diffusion. For all molecules, the S/N increases with the processing time and the rate at which particles pass through the detectors. The latter increases with particle concentration and migration velocity. Lower sample concentrations require a longer processing time to achieve a specified S/N ratio. For smaller analytes, the residence time in the detector becomes a factor and, therefore, so does detector geometry.

Original languageAmerican English
Pages (from-to)5062-5070
Number of pages9
JournalChemistry and Biochemistry Faculty Publications and Presentations
Volume75
Issue number19
DOIs
StatePublished - 3 Sep 2003

EGS Disciplines

  • Chemistry

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