Cyclopamine Bioactivity by Extraction Method from Veratrum californicum

Matthew W. Turner; Roberto Cruz; Jared Mattos; Nic Baughman; Jordan Elwell; Jenny Fothergill; Anna Nielsen; Jessica Brookhouse; Ashton Bartlett; Petr Maleck; Xinzhu Pu; Matthew D. King; Owen M. McDougal

doi:10.1016/j.bmc.2016.06.017

Cyclopamine Bioactivity by Extraction Method from Veratrum californicum

Matthew W. Turner, Roberto Cruz, Jared Mattos, Nic Baughman, Jordan Elwell, Jenny Fothergill, Anna Nielsen, Jessica Brookhouse, Ashton Bartlett, Petr Maleck, Xinzhu Pu, Matthew D. King, Owen M. McDougal

Boise State University

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Veratrum californicum, commonly referred to as corn lily or Californian false hellebore, grows in high mountain meadows and produces the steroidal alkaloid cyclopamine, a potent inhibitor of the Hedgehog (Hh) signaling pathway. The Hh pathway is a crucial regulator of many fundamental processes during vertebrate embryonic development. However, constitutive activation of the Hh pathway contributes to the progression of various cancers. In the present study, a direct correlation was made between the extraction efficiency for cyclopamine from root and rhizome by eight methods, and the associated biological activity in Shh-Light II cells using the Dual-Glo® Luciferase Assay System. Alkaloid recovery ranged from 0.39 to 8.03mg/g, with ethanol soak being determined to be the superior method for obtaining biologically active cyclopamine. Acidic ethanol and supercritical extractions yielded degraded or contaminated cyclopamine with lower antagonistic activity towards Hh signaling.

Original language	American English
Pages (from-to)	3752-7
Number of pages	6
Journal	Bioorganic & Medicinal Chemistry
Volume	24
Issue number	16
DOIs	https://doi.org/10.1016/j.bmc.2016.06.017
State	Published - 15 Aug 2016

Keywords

steroidal alkaloids
hedgehog signaling
Veratrum californicum
cyclopamine

EGS Disciplines

Biology

Access to Document

10.1016/j.bmc.2016.06.017License: Unspecified

https://doi.org/10.1016/j.bmc.2016.06.017

Cite this

@article{1a2f1b1b47564d21b185fdf8bee9986d,

title = "Cyclopamine Bioactivity by Extraction Method from Veratrum californicum",

abstract = "Veratrum californicum, commonly referred to as corn lily or Californian false hellebore, grows in high mountain meadows and produces the steroidal alkaloid cyclopamine, a potent inhibitor of the Hedgehog (Hh) signaling pathway. The Hh pathway is a crucial regulator of many fundamental processes during vertebrate embryonic development. However, constitutive activation of the Hh pathway contributes to the progression of various cancers. In the present study, a direct correlation was made between the extraction efficiency for cyclopamine from root and rhizome by eight methods, and the associated biological activity in Shh-Light II cells using the Dual-Glo{\textregistered} Luciferase Assay System. Alkaloid recovery ranged from 0.39 to 8.03mg/g, with ethanol soak being determined to be the superior method for obtaining biologically active cyclopamine. Acidic ethanol and supercritical extractions yielded degraded or contaminated cyclopamine with lower antagonistic activity towards Hh signaling.",

keywords = "steroidal alkaloids, hedgehog signaling, Veratrum californicum, cyclopamine",

author = "Turner, \{Matthew W.\} and Roberto Cruz and Jared Mattos and Nic Baughman and Jordan Elwell and Jenny Fothergill and Anna Nielsen and Jessica Brookhouse and Ashton Bartlett and Petr Maleck and Xinzhu Pu and King, \{Matthew D.\} and McDougal, \{Owen M.\}",

year = "2016",

month = aug,

day = "15",

doi = "10.1016/j.bmc.2016.06.017",

language = "American English",

volume = "24",

pages = "3752--7",

number = "16",

}

TY - JOUR

T1 - Cyclopamine Bioactivity by Extraction Method from Veratrum californicum

AU - Turner, Matthew W.

AU - Cruz, Roberto

AU - Mattos, Jared

AU - Baughman, Nic

AU - Elwell, Jordan

AU - Fothergill, Jenny

AU - Nielsen, Anna

AU - Brookhouse, Jessica

AU - Bartlett, Ashton

AU - Maleck, Petr

AU - Pu, Xinzhu

AU - King, Matthew D.

AU - McDougal, Owen M.

PY - 2016/8/15

Y1 - 2016/8/15

N2 - Veratrum californicum, commonly referred to as corn lily or Californian false hellebore, grows in high mountain meadows and produces the steroidal alkaloid cyclopamine, a potent inhibitor of the Hedgehog (Hh) signaling pathway. The Hh pathway is a crucial regulator of many fundamental processes during vertebrate embryonic development. However, constitutive activation of the Hh pathway contributes to the progression of various cancers. In the present study, a direct correlation was made between the extraction efficiency for cyclopamine from root and rhizome by eight methods, and the associated biological activity in Shh-Light II cells using the Dual-Glo® Luciferase Assay System. Alkaloid recovery ranged from 0.39 to 8.03mg/g, with ethanol soak being determined to be the superior method for obtaining biologically active cyclopamine. Acidic ethanol and supercritical extractions yielded degraded or contaminated cyclopamine with lower antagonistic activity towards Hh signaling.

AB - Veratrum californicum, commonly referred to as corn lily or Californian false hellebore, grows in high mountain meadows and produces the steroidal alkaloid cyclopamine, a potent inhibitor of the Hedgehog (Hh) signaling pathway. The Hh pathway is a crucial regulator of many fundamental processes during vertebrate embryonic development. However, constitutive activation of the Hh pathway contributes to the progression of various cancers. In the present study, a direct correlation was made between the extraction efficiency for cyclopamine from root and rhizome by eight methods, and the associated biological activity in Shh-Light II cells using the Dual-Glo® Luciferase Assay System. Alkaloid recovery ranged from 0.39 to 8.03mg/g, with ethanol soak being determined to be the superior method for obtaining biologically active cyclopamine. Acidic ethanol and supercritical extractions yielded degraded or contaminated cyclopamine with lower antagonistic activity towards Hh signaling.

KW - steroidal alkaloids

KW - hedgehog signaling

KW - Veratrum californicum

KW - cyclopamine

UR - https://scholarworks.boisestate.edu/bio_facpubs/499

UR - https://doi.org/10.1016/j.bmc.2016.06.017

U2 - 10.1016/j.bmc.2016.06.017

DO - 10.1016/j.bmc.2016.06.017

M3 - Article

C2 - 27338657

VL - 24

SP - 3752

EP - 3757

JO - Bioorganic & Medicinal Chemistry

JF - Bioorganic & Medicinal Chemistry

IS - 16

ER -

Cyclopamine Bioactivity by Extraction Method from Veratrum californicum

Abstract

Keywords

EGS Disciplines

Access to Document

Other files and links

Fingerprint

Cite this