Detecting single stranded DNA with a solid state nanopore

Daniel Fologea; Marc Gershow; Bradley Ledden; David S. McNabb; Jene A. Golovchenko; Jiali Li

doi:10.1021/nl051199m

Detecting single stranded DNA with a solid state nanopore

Daniel Fologea, Marc Gershow, Bradley Ledden, David S. McNabb, Jene A. Golovchenko, Jiali Li

Physics Department

Research output: Contribution to journal › Article › peer-review

382 Scopus citations

Abstract

Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.

Original language	English
Pages (from-to)	1905-1909
Number of pages	5
Journal	Nano Letters
Volume	5
Issue number	10
DOIs	https://doi.org/10.1021/nl051199m
State	Published - Oct 2005

Keywords

DNA, Single-Stranded/analysis
Electrochemistry/methods
Nanotechnology/methods
Nucleic Acid Conformation

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10.1021/nl051199m

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abstract = "Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.",

keywords = "DNA, Single-Stranded/analysis, Electrochemistry/methods, Nanotechnology/methods, Nucleic Acid Conformation",

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AU - Fologea, Daniel

AU - Gershow, Marc

AU - Ledden, Bradley

AU - McNabb, David S.

AU - Golovchenko, Jene A.

AU - Li, Jiali

PY - 2005/10

Y1 - 2005/10

N2 - Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.

AB - Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.

KW - DNA, Single-Stranded/analysis

KW - Electrochemistry/methods

KW - Nanotechnology/methods

KW - Nucleic Acid Conformation

UR - https://www.scopus.com/pages/publications/27644483208

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DO - 10.1021/nl051199m

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VL - 5

SP - 1905

EP - 1909

JO - Nano Letters

JF - Nano Letters

IS - 10

ER -

Detecting single stranded DNA with a solid state nanopore

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Keywords

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