Development of a bacterial nanoparticle vaccine

Carlos Gamazo; Javier Ochoa-Repáraz; Ibai Tamayo; Ana Camacho; Juan M. Irache

doi:10.1007/978-1-4939-1625-2_10

Development of a bacterial nanoparticle vaccine

Carlos Gamazo, Javier Ochoa-Repáraz, Ibai Tamayo, Ana Camacho, Juan M. Irache

Department of Biological Sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN ®).

Original language	English
Pages (from-to)	139-149
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1225
DOIs	https://doi.org/10.1007/978-1-4939-1625-2_10
State	Published - 2015

Keywords

Acellular vaccine
Gram-negative bacteria
Nanoparticles
OMV
Outer membrane
Salmonella
Vaccine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-1625-2_10

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title = "Development of a bacterial nanoparticle vaccine",

abstract = "A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN {\textregistered}).",

keywords = "Acellular vaccine, Gram-negative bacteria, Nanoparticles, OMV, Outer membrane, Salmonella, Vaccine",

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AU - Gamazo, Carlos

AU - Ochoa-Repáraz, Javier

AU - Tamayo, Ibai

AU - Camacho, Ana

AU - Irache, Juan M.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN ®).

AB - A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN ®).

KW - Acellular vaccine

KW - Gram-negative bacteria

KW - Nanoparticles

KW - OMV

KW - Outer membrane

KW - Salmonella

KW - Vaccine

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AN - SCOPUS:84954576023

SN - 1064-3745

VL - 1225

SP - 139

EP - 149

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Development of a bacterial nanoparticle vaccine

Abstract

Keywords

UN SDGs

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