Development of a bacterial nanoparticle vaccine

Carlos Gamazo; Javier Ochoa-Repáraz; Ibai Tamayo; Ana Camacho; Juan M. Irache

doi:10.1007/978-1-4939-1625-2_10

Development of a bacterial nanoparticle vaccine

Carlos Gamazo, Javier Ochoa-Repáraz, Ibai Tamayo, Ana Camacho, Juan M. Irache

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN ®).

Original language	English
Title of host publication	Salmonella
Subtitle of host publication	Methods and Protocols: Second Edition
Publisher	Springer New York
Pages	139-149
Number of pages	11
ISBN (Electronic)	9781493916252
ISBN (Print)	9781493916245
DOIs	https://doi.org/10.1007/978-1-4939-1625-2_10
State	Published - 24 Sep 2014

Keywords

Acellular vaccine
Gram-negative bacteria
Nanoparticles
OMV
Outer membrane
Salmonella
Vaccine

EGS Disciplines

Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-1625-2_10

Cite this

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abstract = "A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN {\textregistered}).",

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T1 - Development of a bacterial nanoparticle vaccine

AU - Gamazo, Carlos

AU - Ochoa-Repáraz, Javier

AU - Tamayo, Ibai

AU - Camacho, Ana

AU - Irache, Juan M.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015. All rights are reserved.

PY - 2014/9/24

Y1 - 2014/9/24

N2 - A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN ®).

AB - A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN ®).

KW - Acellular vaccine

KW - Gram-negative bacteria

KW - Nanoparticles

KW - OMV

KW - Outer membrane

KW - Salmonella

KW - Vaccine

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M3 - Chapter

AN - SCOPUS:84921765519

SN - 9781493916245

SP - 139

EP - 149

BT - Salmonella

PB - Springer New York

ER -

Development of a bacterial nanoparticle vaccine

Abstract

Keywords

EGS Disciplines

UN SDGs

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