Diagnostic potential for human malignancies of bacterially produced HTLV-I envelope protein

Kenneth P. Samuel; James A. Lautenberger; Cheryl L. Jorcyk; Steven Josephs; Flossie Wong-Staal; Takis S. Papas

doi:10.1126/science.6208612

Diagnostic potential for human malignancies of bacterially produced HTLV-I envelope protein

Kenneth P. Samuel, James A. Lautenberger, Cheryl L. Jorcyk, Steven Josephs, Flossie Wong-Staal, Takis S. Papas

Department of Biological Sciences

National Institutes of Health

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Two regions of the gene for the human T-cell leukemia virus subgroup I (HTLV-I) envelope were expressed in Escherichia coli by use of the vector pJLA16. One corresponds to the carboxyl terminal region of the major envelope protein p46, and the other corresponds to the transmembrane protein p21E. Reactivity of the expressed protein with human serum was tested by the Western blot procedure. Each of 11 sera tested that had been shown to contain antibodies to HTLV-I or HTLV-II by an enzyme-linked immunosorbent assay recognized the bacterially synthesized envelope proteins. There was no reaction detected when 17 control sera were tested. This system will be useful for large-scale seroepidemiological surveys for HTLV-I and related human retroviruses.

Original language	English
Pages (from-to)	1094-1097
Number of pages	4
Journal	Science
Volume	226
Issue number	4678
DOIs	https://doi.org/10.1126/science.6208612
State	Published - 30 Nov 1984

Keywords

Acquired Immunodeficiency Syndrome/diagnosis
Base Sequence
DNA Restriction Enzymes
Deltaretrovirus/genetics
Epitopes/analysis
Escherichia coli/genetics
Genes, Viral
Genetic Vectors
Humans
Male
Neoplasms/diagnosis
Plasmids
Viral Envelope Proteins/genetics

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10.1126/science.6208612

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title = "Diagnostic potential for human malignancies of bacterially produced HTLV-I envelope protein",

abstract = "Two regions of the gene for the human T-cell leukemia virus subgroup I (HTLV-I) envelope were expressed in Escherichia coli by use of the vector pJLA16. One corresponds to the carboxyl terminal region of the major envelope protein p46, and the other corresponds to the transmembrane protein p21E. Reactivity of the expressed protein with human serum was tested by the Western blot procedure. Each of 11 sera tested that had been shown to contain antibodies to HTLV-I or HTLV-II by an enzyme-linked immunosorbent assay recognized the bacterially synthesized envelope proteins. There was no reaction detected when 17 control sera were tested. This system will be useful for large-scale seroepidemiological surveys for HTLV-I and related human retroviruses.",

keywords = "Acquired Immunodeficiency Syndrome/diagnosis, Base Sequence, DNA Restriction Enzymes, Deltaretrovirus/genetics, Epitopes/analysis, Escherichia coli/genetics, Genes, Viral, Genetic Vectors, Humans, Male, Neoplasms/diagnosis, Plasmids, Viral Envelope Proteins/genetics",

author = "Samuel, {Kenneth P.} and Lautenberger, {James A.} and Jorcyk, {Cheryl L.} and Steven Josephs and Flossie Wong-Staal and Papas, {Takis S.}",

year = "1984",

month = nov,

day = "30",

doi = "10.1126/science.6208612",

language = "English",

volume = "226",

pages = "1094--1097",

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T1 - Diagnostic potential for human malignancies of bacterially produced HTLV-I envelope protein

AU - Samuel, Kenneth P.

AU - Lautenberger, James A.

AU - Jorcyk, Cheryl L.

AU - Josephs, Steven

AU - Wong-Staal, Flossie

AU - Papas, Takis S.

PY - 1984/11/30

Y1 - 1984/11/30

N2 - Two regions of the gene for the human T-cell leukemia virus subgroup I (HTLV-I) envelope were expressed in Escherichia coli by use of the vector pJLA16. One corresponds to the carboxyl terminal region of the major envelope protein p46, and the other corresponds to the transmembrane protein p21E. Reactivity of the expressed protein with human serum was tested by the Western blot procedure. Each of 11 sera tested that had been shown to contain antibodies to HTLV-I or HTLV-II by an enzyme-linked immunosorbent assay recognized the bacterially synthesized envelope proteins. There was no reaction detected when 17 control sera were tested. This system will be useful for large-scale seroepidemiological surveys for HTLV-I and related human retroviruses.

AB - Two regions of the gene for the human T-cell leukemia virus subgroup I (HTLV-I) envelope were expressed in Escherichia coli by use of the vector pJLA16. One corresponds to the carboxyl terminal region of the major envelope protein p46, and the other corresponds to the transmembrane protein p21E. Reactivity of the expressed protein with human serum was tested by the Western blot procedure. Each of 11 sera tested that had been shown to contain antibodies to HTLV-I or HTLV-II by an enzyme-linked immunosorbent assay recognized the bacterially synthesized envelope proteins. There was no reaction detected when 17 control sera were tested. This system will be useful for large-scale seroepidemiological surveys for HTLV-I and related human retroviruses.

KW - Acquired Immunodeficiency Syndrome/diagnosis

KW - Base Sequence

KW - DNA Restriction Enzymes

KW - Deltaretrovirus/genetics

KW - Epitopes/analysis

KW - Escherichia coli/genetics

KW - Genes, Viral

KW - Genetic Vectors

KW - Humans

KW - Male

KW - Neoplasms/diagnosis

KW - Plasmids

KW - Viral Envelope Proteins/genetics

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U2 - 10.1126/science.6208612

DO - 10.1126/science.6208612

M3 - Article

C2 - 6208612

AN - SCOPUS:0021748477

SN - 0036-8075

VL - 226

SP - 1094

EP - 1097

JO - Science

JF - Science

IS - 4678

ER -

Diagnostic potential for human malignancies of bacterially produced HTLV-I envelope protein

Abstract

Keywords

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