Dielectrophoretic trapping of DNA origami

Anton Kuzyk, Bernard Yurke, J. Jussi Toppari, Veikko Linko, Päivi Törmä

Research output: Contribution to journalArticlepeer-review

92 Scopus citations

Abstract

The development of a dielectrophoresis-based method for tapping DNA orgami structures on the chip, obtained by self-assembly of long single-stranded DNA, is described. Dielectrophoresis (DEP) involves movement of polarizable particle such as, cells, viruses, and long DNA molecules in a nonuniform electric filed. The experiment involved the use of a rectangular and a disk-shaped DNA orgami structures for the tapping procedure. Thermal annealing was carried out for fabricating them and the structures so obtained were ligated using T4 DNA ligase. The parameters of frequencies and voltage were carefully optimized during dielectrophoresis in order to maximize the yield of the orgami structures. Bottom-up and top-down approaches were successfully blended and lithography was used for fabricating the electrodes. DNA orgami structures were obtained at a concentration of about 1 nm in the final solution.

Original languageEnglish
Pages (from-to)447-450
Number of pages4
JournalSmall
Volume4
Issue number4
DOIs
StatePublished - Apr 2008

Keywords

  • Dielectrophoresis
  • DNA self-assembly
  • Nanoelectrodes
  • Nanomanipulation

Fingerprint

Dive into the research topics of 'Dielectrophoretic trapping of DNA origami'. Together they form a unique fingerprint.

Cite this