Abstract
The development of a dielectrophoresis-based method for tapping DNA orgami structures on the chip, obtained by self-assembly of long single-stranded DNA, is described. Dielectrophoresis (DEP) involves movement of polarizable particle such as, cells, viruses, and long DNA molecules in a nonuniform electric filed. The experiment involved the use of a rectangular and a disk-shaped DNA orgami structures for the tapping procedure. Thermal annealing was carried out for fabricating them and the structures so obtained were ligated using T4 DNA ligase. The parameters of frequencies and voltage were carefully optimized during dielectrophoresis in order to maximize the yield of the orgami structures. Bottom-up and top-down approaches were successfully blended and lithography was used for fabricating the electrodes. DNA orgami structures were obtained at a concentration of about 1 nm in the final solution.
Original language | English |
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Pages (from-to) | 447-450 |
Number of pages | 4 |
Journal | Small |
Volume | 4 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2008 |
Keywords
- Dielectrophoresis
- DNA self-assembly
- Nanoelectrodes
- Nanomanipulation