Directed Solid Phase Peptide Synthesis of alpha-Conotoxin MII

Jenny Fothergill, Vanessa Bowman, Matt Turner, Matt King, Owen McDougal

Research output: Contribution to conferencePoster

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Abstract

Conotoxins—short, disulfide-rich peptides found in the venom of cone snails—exhibit unprecedented receptor binding selectivity. α-Conotoxin MII (α-CTxMII) targets the α 3 β 2 -nicotinic acetylcholine receptor (nAChR) isoform; studying the interaction of this peptide with nAChRs provides an understanding of binding determinants that may benefit therapeutic development for diseases like Alzheimer’s and Parkinson’s. Linear peptide was synthesized on solid support RINK resin by automated peptide synthesis followed by cleavage from resin and subsequent oxidative folding to form disulfide bridges. Mass spectral analysis validated proper synthesis and initial fold formation. QTOF-MS (m/z): [M + H] + calculated for α-CTxMII, 1856.8; found, 928.9, 619.6 which correspond to the doubly and triply charged molecular ions. Next the peptide will be purified by chromatography and again verified using mass spectrometry. Finished product will be used to validate the applicability of PC-12 cells for expression of α 3 β 2 -nAChRs.

Original languageAmerican English
StatePublished - 1 Jul 2016
EventIdaho Conference on Undergraduate Research 2016 - Boise State University, Boise, United States
Duration: 1 Jul 2016 → …

Conference

ConferenceIdaho Conference on Undergraduate Research 2016
Abbreviated titleICUR 2016
Country/TerritoryUnited States
CityBoise
Period1/07/16 → …

EGS Disciplines

  • Chemistry

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