Effects of Yariv phenylglycosides on Rosa cell suspensions: Evidence for the involvement of arabinogalactan-proteins in cell proliferation

Marcelo D. Serpe, Eugene A. Nothnagel

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172 Scopus citations

Abstract

Treatment of 'Paul's Scarlet rose (Rosa sp.) cell suspensions with β-D-glucosyl Yariv phenylglycoside (β-D-Glc)3, a chromophoric molecule that selectively binds arabinogalactan-proteins (AGPs), caused inhibition of cell growth in a concentration-dependent manner, with complete inhibition of growth occurring at 50 μM (β-D-Glc)3 in the culture medium. Growth was not inhibited by either α-D-galactosyl or β-D-mannosyl Yariv phenylglycosides which do not bind AGPs. Staining of cells with fluorescein diacetate indicated that (β-D-Glc)3 did not affect cell viability. Upon transfer of 50 μM (β-D-Glc)3-treated cells to control conditions, cell growth recovered with a time-course similar to that of control cells. Cell sizes in control and (β-D-Glc)3-treated cultures were similar, indicating that the mechanism of growth inhibition by (β-D-Glc)3 involved suppression of cell division. Two different analyses of (β-D-Glc)3-treated cells both showed that approximately 95% of the bound (β-D-Glc)3 was in the cell wall. Molecules that bound (β-D-Glc)3 were extracted from the cell wall and were identified as AGPs, as judged by their carbohydrate and amino acid compositions.

Original languageEnglish
Pages (from-to)542-550
Number of pages9
JournalPlanta
Volume193
Issue number4
DOIs
StatePublished - May 1994

Keywords

  • (β-d-glucosyl) Yariv phenylglycoside
  • Arabinogalactan-protein
  • Cell proliferation
  • Cell wall
  • Plasma membrane
  • Rosa

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