TY - JOUR
T1 - Effects of Yariv phenylglycosides on Rosa cell suspensions
T2 - Evidence for the involvement of arabinogalactan-proteins in cell proliferation
AU - Serpe, Marcelo D.
AU - Nothnagel, Eugene A.
PY - 1994/5
Y1 - 1994/5
N2 - Treatment of 'Paul's Scarlet rose (Rosa sp.) cell suspensions with β-D-glucosyl Yariv phenylglycoside (β-D-Glc)3, a chromophoric molecule that selectively binds arabinogalactan-proteins (AGPs), caused inhibition of cell growth in a concentration-dependent manner, with complete inhibition of growth occurring at 50 μM (β-D-Glc)3 in the culture medium. Growth was not inhibited by either α-D-galactosyl or β-D-mannosyl Yariv phenylglycosides which do not bind AGPs. Staining of cells with fluorescein diacetate indicated that (β-D-Glc)3 did not affect cell viability. Upon transfer of 50 μM (β-D-Glc)3-treated cells to control conditions, cell growth recovered with a time-course similar to that of control cells. Cell sizes in control and (β-D-Glc)3-treated cultures were similar, indicating that the mechanism of growth inhibition by (β-D-Glc)3 involved suppression of cell division. Two different analyses of (β-D-Glc)3-treated cells both showed that approximately 95% of the bound (β-D-Glc)3 was in the cell wall. Molecules that bound (β-D-Glc)3 were extracted from the cell wall and were identified as AGPs, as judged by their carbohydrate and amino acid compositions.
AB - Treatment of 'Paul's Scarlet rose (Rosa sp.) cell suspensions with β-D-glucosyl Yariv phenylglycoside (β-D-Glc)3, a chromophoric molecule that selectively binds arabinogalactan-proteins (AGPs), caused inhibition of cell growth in a concentration-dependent manner, with complete inhibition of growth occurring at 50 μM (β-D-Glc)3 in the culture medium. Growth was not inhibited by either α-D-galactosyl or β-D-mannosyl Yariv phenylglycosides which do not bind AGPs. Staining of cells with fluorescein diacetate indicated that (β-D-Glc)3 did not affect cell viability. Upon transfer of 50 μM (β-D-Glc)3-treated cells to control conditions, cell growth recovered with a time-course similar to that of control cells. Cell sizes in control and (β-D-Glc)3-treated cultures were similar, indicating that the mechanism of growth inhibition by (β-D-Glc)3 involved suppression of cell division. Two different analyses of (β-D-Glc)3-treated cells both showed that approximately 95% of the bound (β-D-Glc)3 was in the cell wall. Molecules that bound (β-D-Glc)3 were extracted from the cell wall and were identified as AGPs, as judged by their carbohydrate and amino acid compositions.
KW - (β-d-glucosyl) Yariv phenylglycoside
KW - Arabinogalactan-protein
KW - Cell proliferation
KW - Cell wall
KW - Plasma membrane
KW - Rosa
UR - http://www.scopus.com/inward/record.url?scp=0028155874&partnerID=8YFLogxK
U2 - 10.1007/BF02411560
DO - 10.1007/BF02411560
M3 - Article
AN - SCOPUS:0028155874
SN - 0032-0935
VL - 193
SP - 542
EP - 550
JO - Planta
JF - Planta
IS - 4
ER -