TY - JOUR
T1 - Exercise attenuates α-adrenergic-receptor responsiveness in skeletal muscle vasculature
AU - Buckwalter, J. B.
AU - Naik, J. S.
AU - Valic, Z.
AU - Clifford, P. S.
PY - 2001
Y1 - 2001
N2 - Attenuation of sympathetic vasoconstriction (sympatholysis) in working muscles during dynamic exercise is controversial. A potential mechanism is a reduction in α-adrenergic-receptor responsiveness. The purpose of this study was to examine α1- and α2-adrenergic-receptor-mediated vasoconstriction in resting and exercising skeletal muscle using intra-arterial infusions of selective agonists. Thirteen mongrel dogs were instrumented chronically with flow probes on the external iliac arteries of both hindlimbs and a catheter in one femoral artery. The selective α1-adrenergic agonist (phenylephrine) or the selective α2-adrenergic agonist (clonidine) was infused as a bolus into the femoral artery catheter at rest and during mild and heavy exercise. Intra-arterial infusions of phenylephrine elicited reductions in vascular conductance of 76 ± 4, 71 ± 5, and 31 ± 2% at rest, 3 miles/h, and 6 miles/h and 10% grade, respectively. Intra-arterial clonidine reduced vascular conductance by 81 ± 5, 49 ± 4, and 14 ± 2%, respectively. The response to intra-arterial infusion of clonidine was unaffected by surgical sympathetic denervation. Agonist infusion did not affect either systemic blood pressure, heart rate, or blood flow in the contralateral iliac artery. α1-Adrenergic-receptor responsiveness was attenuated during heavy exercise. In contrast, α2-adrenergic-receptor responsiveness was attenuated even at a mild exercise intensity. These results suggest that the mechanism of exercise sympatholysis may involve reductions in postsynaptic α-adrenergic-receptor responsiveness.
AB - Attenuation of sympathetic vasoconstriction (sympatholysis) in working muscles during dynamic exercise is controversial. A potential mechanism is a reduction in α-adrenergic-receptor responsiveness. The purpose of this study was to examine α1- and α2-adrenergic-receptor-mediated vasoconstriction in resting and exercising skeletal muscle using intra-arterial infusions of selective agonists. Thirteen mongrel dogs were instrumented chronically with flow probes on the external iliac arteries of both hindlimbs and a catheter in one femoral artery. The selective α1-adrenergic agonist (phenylephrine) or the selective α2-adrenergic agonist (clonidine) was infused as a bolus into the femoral artery catheter at rest and during mild and heavy exercise. Intra-arterial infusions of phenylephrine elicited reductions in vascular conductance of 76 ± 4, 71 ± 5, and 31 ± 2% at rest, 3 miles/h, and 6 miles/h and 10% grade, respectively. Intra-arterial clonidine reduced vascular conductance by 81 ± 5, 49 ± 4, and 14 ± 2%, respectively. The response to intra-arterial infusion of clonidine was unaffected by surgical sympathetic denervation. Agonist infusion did not affect either systemic blood pressure, heart rate, or blood flow in the contralateral iliac artery. α1-Adrenergic-receptor responsiveness was attenuated during heavy exercise. In contrast, α2-adrenergic-receptor responsiveness was attenuated even at a mild exercise intensity. These results suggest that the mechanism of exercise sympatholysis may involve reductions in postsynaptic α-adrenergic-receptor responsiveness.
KW - Autonomic nervous system
KW - Blood flow
KW - Dogs
KW - Sympatholysis
KW - Vasoconstriction
UR - http://www.scopus.com/inward/record.url?scp=0035164434&partnerID=8YFLogxK
U2 - 10.1152/jappl.2001.90.1.172
DO - 10.1152/jappl.2001.90.1.172
M3 - Article
C2 - 11133908
AN - SCOPUS:0035164434
SN - 8750-7587
VL - 90
SP - 172
EP - 178
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
IS - 1
ER -