TY - JOUR
T1 - Expression, purification, crystallization and preliminary x-ray analysis of escherichia coli 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidase
AU - Lee, J. E.
AU - Cornell, K. A.
AU - Riscoe, M. K.
AU - Lynne Howell, P.
PY - 2001
Y1 - 2001
N2 - A recombinant form of Escherichia coli 5'-methylthioadenosine/ S-adenosylhomocysteine nucleosidase (E.C. 3.2.2.9) has been purified to homogeneity and crystallized using the hanging-drop vapour-diffusion technique. While several different crystallization conditions were obtained, only one set of conditions yielded crystals suitable for X-ray diffraction analysis. These crystals grow as diamond-shaped wedges, with unit-cell parameters a = 50.92, b = 133.99, c = 70.88 Å, a = β = γ = 90°. The crystals belong to space group P21212 and diffract to a minimum d spacing of 2.3 Å on a MAR345 image plate with a Rigaku RU-200 rotating-anode X-ray generator. On the basis of density calculations, two monomers are predicted per asymmetric unit (Matthews coefficient, VM = 2.37 Å3 Da-1), with a solvent content of 48%.
AB - A recombinant form of Escherichia coli 5'-methylthioadenosine/ S-adenosylhomocysteine nucleosidase (E.C. 3.2.2.9) has been purified to homogeneity and crystallized using the hanging-drop vapour-diffusion technique. While several different crystallization conditions were obtained, only one set of conditions yielded crystals suitable for X-ray diffraction analysis. These crystals grow as diamond-shaped wedges, with unit-cell parameters a = 50.92, b = 133.99, c = 70.88 Å, a = β = γ = 90°. The crystals belong to space group P21212 and diffract to a minimum d spacing of 2.3 Å on a MAR345 image plate with a Rigaku RU-200 rotating-anode X-ray generator. On the basis of density calculations, two monomers are predicted per asymmetric unit (Matthews coefficient, VM = 2.37 Å3 Da-1), with a solvent content of 48%.
UR - http://www.scopus.com/inward/record.url?scp=0035180468&partnerID=8YFLogxK
U2 - 10.1107/S0907444900014669
DO - 10.1107/S0907444900014669
M3 - Article
C2 - 11134941
AN - SCOPUS:0035180468
SN - 0907-4449
VL - 57
SP - 150
EP - 152
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 1
ER -