Farnesol Brain Transcriptomics in CNS Inflammatory Demyelination

William J. Doyle; Dana Walters; Xutong Shi; Kristina Hoffman; Krisztian Magori; Jean-Baptiste Roullet; Javier Ochoa-Reparaz

doi:10.1016/j.clim.2023.109752

Farnesol Brain Transcriptomics in CNS Inflammatory Demyelination

William J. Doyle, Dana Walters, Xutong Shi, Kristina Hoffman, Krisztian Magori, Jean-Baptiste Roullet, Javier Ochoa-Reparaz

Biological Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

BACKGROUND: Farnesol (FOL) prevents the onset of experimental autoimmune encephalomyelitis (EAE), a murine model of multiple sclerosis (MS).

OBJECTIVE: We examined the transcriptomic profile of the brains of EAE mice treated with daily oral FOL using next-generation sequencing (RNA-seq).

METHODS: Transcriptomics from whole brains of treated and untreated EAE mice at the peak of EAE was performed.

RESULTS: EAE-induced mice, compared to naïve, healthy mice, overall showed increased expression in pathways for immune response, as well as an increased cytokine signaling pathway, with downregulation of cellular stress proteins. FOL downregulates pro-inflammatory pathways and attenuates the immune response in EAE. FOL downregulated the expression of genes involved in misfolded protein response, MAPK activation/signaling, and pro-inflammatory response.

CONCLUSION: This study provides insight into the molecular impact of FOL in the brain and identifies potential therapeutic targets of the isoprenoid pathway in MS patients.

Original language	American English
Article number	109752
Journal	Clinical Immunology
Volume	255
DOIs	https://doi.org/10.1016/j.clim.2023.109752
State	Published - Oct 2023

Keywords

Brain
EAE
Farnesol
Isoprenoids
Transcriptomics

EGS Disciplines

Biology

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10.1016/j.clim.2023.109752

https://scholarworks.boisestate.edu/bio_facpubs/806License: CC BY-NC-ND

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@article{e3900fec6af84337aa252f25c391b8d8,

title = "Farnesol Brain Transcriptomics in CNS Inflammatory Demyelination",

abstract = "BACKGROUND: Farnesol (FOL) prevents the onset of experimental autoimmune encephalomyelitis (EAE), a murine model of multiple sclerosis (MS).OBJECTIVE: We examined the transcriptomic profile of the brains of EAE mice treated with daily oral FOL using next-generation sequencing (RNA-seq).METHODS: Transcriptomics from whole brains of treated and untreated EAE mice at the peak of EAE was performed.RESULTS: EAE-induced mice, compared to na{\"i}ve, healthy mice, overall showed increased expression in pathways for immune response, as well as an increased cytokine signaling pathway, with downregulation of cellular stress proteins. FOL downregulates pro-inflammatory pathways and attenuates the immune response in EAE. FOL downregulated the expression of genes involved in misfolded protein response, MAPK activation/signaling, and pro-inflammatory response.CONCLUSION: This study provides insight into the molecular impact of FOL in the brain and identifies potential therapeutic targets of the isoprenoid pathway in MS patients.",

keywords = "Brain, EAE, Farnesol, Isoprenoids, Transcriptomics",

author = "Doyle, \{William J.\} and Dana Walters and Xutong Shi and Kristina Hoffman and Krisztian Magori and Jean-Baptiste Roullet and Javier Ochoa-Reparaz",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier Inc.",

year = "2023",

month = oct,

doi = "10.1016/j.clim.2023.109752",

language = "American English",

volume = "255",

}

TY - JOUR

T1 - Farnesol Brain Transcriptomics in CNS Inflammatory Demyelination

AU - Doyle, William J.

AU - Walters, Dana

AU - Shi, Xutong

AU - Hoffman, Kristina

AU - Magori, Krisztian

AU - Roullet, Jean-Baptiste

AU - Ochoa-Reparaz, Javier

PY - 2023/10

Y1 - 2023/10

N2 - BACKGROUND: Farnesol (FOL) prevents the onset of experimental autoimmune encephalomyelitis (EAE), a murine model of multiple sclerosis (MS).OBJECTIVE: We examined the transcriptomic profile of the brains of EAE mice treated with daily oral FOL using next-generation sequencing (RNA-seq).METHODS: Transcriptomics from whole brains of treated and untreated EAE mice at the peak of EAE was performed.RESULTS: EAE-induced mice, compared to naïve, healthy mice, overall showed increased expression in pathways for immune response, as well as an increased cytokine signaling pathway, with downregulation of cellular stress proteins. FOL downregulates pro-inflammatory pathways and attenuates the immune response in EAE. FOL downregulated the expression of genes involved in misfolded protein response, MAPK activation/signaling, and pro-inflammatory response.CONCLUSION: This study provides insight into the molecular impact of FOL in the brain and identifies potential therapeutic targets of the isoprenoid pathway in MS patients.

AB - BACKGROUND: Farnesol (FOL) prevents the onset of experimental autoimmune encephalomyelitis (EAE), a murine model of multiple sclerosis (MS).OBJECTIVE: We examined the transcriptomic profile of the brains of EAE mice treated with daily oral FOL using next-generation sequencing (RNA-seq).METHODS: Transcriptomics from whole brains of treated and untreated EAE mice at the peak of EAE was performed.RESULTS: EAE-induced mice, compared to naïve, healthy mice, overall showed increased expression in pathways for immune response, as well as an increased cytokine signaling pathway, with downregulation of cellular stress proteins. FOL downregulates pro-inflammatory pathways and attenuates the immune response in EAE. FOL downregulated the expression of genes involved in misfolded protein response, MAPK activation/signaling, and pro-inflammatory response.CONCLUSION: This study provides insight into the molecular impact of FOL in the brain and identifies potential therapeutic targets of the isoprenoid pathway in MS patients.

KW - Brain

KW - EAE

KW - Farnesol

KW - Isoprenoids

KW - Transcriptomics

UR - https://www.scopus.com/pages/publications/85171350063

U2 - 10.1016/j.clim.2023.109752

DO - 10.1016/j.clim.2023.109752

M3 - Article

C2 - 37673223

SN - 1521-6616

VL - 255

JO - Clinical Immunology

JF - Clinical Immunology

M1 - 109752

ER -

Farnesol Brain Transcriptomics in CNS Inflammatory Demyelination

Abstract

Keywords

EGS Disciplines

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