TY - JOUR
T1 - FimZ binds the Salmonella typhimurium fimA promoter region and may regulate its own expression with FimY
AU - Yeh, Kuang Sheng
AU - Tinker, Juliette K.
AU - Clegg, Steven
PY - 2002
Y1 - 2002
N2 - The FimZ protein, an activator of FimA production in Salmonella typhimurium, acts in conjunction with FimY to facilitate the expression of type 1 fimbriae. The predicted amino acid sequence of FimZ suggests that this protein may be a DNA-binding protein related to BvgA, a sensory regulator of virulence gene expression in Bordetella pertussis. Purification of FimZ following overexpression of the protein by a strong inducible promoter and gel mobility shift assays confirm that FimZ is a 25-kDa polypeptide that binds to the promoter region of fimA. The region of DNA protected from DNase I digestion by FimZ binding is located between 47 and 98 nucleotides upstream from the fimA transcription initiation site. This region possesses a pair of 7-base pair tandem repeats, of which at least one is necessary for FimZ binding. One copy of the 7-base pair sequence is also located in the fimZ promoter region. In addition, expression from a fimZ-lacZ reporter construct confirms that FimZ plays a role in its own expression. Both FimZ and FimY are required for high-level expression of FimZ, which suggests that these two fimbrial proteins are involved in regulating both FimA and FimZ.
AB - The FimZ protein, an activator of FimA production in Salmonella typhimurium, acts in conjunction with FimY to facilitate the expression of type 1 fimbriae. The predicted amino acid sequence of FimZ suggests that this protein may be a DNA-binding protein related to BvgA, a sensory regulator of virulence gene expression in Bordetella pertussis. Purification of FimZ following overexpression of the protein by a strong inducible promoter and gel mobility shift assays confirm that FimZ is a 25-kDa polypeptide that binds to the promoter region of fimA. The region of DNA protected from DNase I digestion by FimZ binding is located between 47 and 98 nucleotides upstream from the fimA transcription initiation site. This region possesses a pair of 7-base pair tandem repeats, of which at least one is necessary for FimZ binding. One copy of the 7-base pair sequence is also located in the fimZ promoter region. In addition, expression from a fimZ-lacZ reporter construct confirms that FimZ plays a role in its own expression. Both FimZ and FimY are required for high-level expression of FimZ, which suggests that these two fimbrial proteins are involved in regulating both FimA and FimZ.
KW - Activator
KW - Salmonella typhimurium
KW - Type 1 fimbriae
UR - http://www.scopus.com/inward/record.url?scp=0036154824&partnerID=8YFLogxK
U2 - 10.1111/j.1348-0421.2002.tb02670.x
DO - 10.1111/j.1348-0421.2002.tb02670.x
M3 - Article
C2 - 11911183
AN - SCOPUS:0036154824
SN - 0385-5600
VL - 46
SP - 1
EP - 10
JO - Microbiology and Immunology
JF - Microbiology and Immunology
IS - 1
ER -