TY - JOUR
T1 - Fluorescent Dye Encapsulated ZnO Particles with Cell-Specific Toxicity for Potential Use in Biomedical Applications
AU - Wang, Hua
AU - Wingett, Denise G.
AU - Engelhard, Mark H.
AU - Feris, Kevin P.
AU - Reddy, K. M.
AU - Turner, Paul
AU - Layne, Janet
AU - Hanley, Cory
AU - Bell, Jason
AU - Tenne, Dmitri
AU - Wang, Chongmin
AU - Punnoose, Alex
PY - 2009/1
Y1 - 2009/1
N2 - Fluorescein isothiocyanate (FITC)-encapsulated SiO2 core-shell particles with a nanoscale ZnO finishing layer have been synthesized for the first time as multifunctional "smart" nanostructures. Detailed characterization studies confirmed the formation of an outer ZnO layer on the SiO2-FITC core. These ∼200 nm sized particles showed promise toward cell imaging and cellular uptake studies using the bacterium Escherichia coli and Jurkat cancer cells, respectively. The FITC encapsulated ZnO particles demonstrated excellent selectivity in preferentially killing Jurkat cancer cells with minimal toxicity to normal primary immune cells (18% and 75% viability remaining, respectively, after exposure to 60 μg/ml) and inhibited the growth of both gram-positive and gram-negative bacteria at concentrations ≥250-500 μg/ml (for Staphylococcus aureus and Escherichia coli, respectively). These results indicate that the novel FITC encapsulated multifunctional particles with nanoscale ZnO surface layer can be used as smart nanostructures for particle tracking, cell imaging, antibacterial treatments and cancer therapy.
AB - Fluorescein isothiocyanate (FITC)-encapsulated SiO2 core-shell particles with a nanoscale ZnO finishing layer have been synthesized for the first time as multifunctional "smart" nanostructures. Detailed characterization studies confirmed the formation of an outer ZnO layer on the SiO2-FITC core. These ∼200 nm sized particles showed promise toward cell imaging and cellular uptake studies using the bacterium Escherichia coli and Jurkat cancer cells, respectively. The FITC encapsulated ZnO particles demonstrated excellent selectivity in preferentially killing Jurkat cancer cells with minimal toxicity to normal primary immune cells (18% and 75% viability remaining, respectively, after exposure to 60 μg/ml) and inhibited the growth of both gram-positive and gram-negative bacteria at concentrations ≥250-500 μg/ml (for Staphylococcus aureus and Escherichia coli, respectively). These results indicate that the novel FITC encapsulated multifunctional particles with nanoscale ZnO surface layer can be used as smart nanostructures for particle tracking, cell imaging, antibacterial treatments and cancer therapy.
UR - http://www.scopus.com/inward/record.url?scp=58549115045&partnerID=8YFLogxK
UR - https://scholarworks.boisestate.edu/physics_facpubs/49
U2 - 10.1007/s10856-008-3541-z
DO - 10.1007/s10856-008-3541-z
M3 - Article
C2 - 18651111
SN - 0957-4530
VL - 20
SP - 11
EP - 22
JO - Journal of Materials Science: Materials in Medicine
JF - Journal of Materials Science: Materials in Medicine
IS - 1
ER -