Force-Feedback High-Speed Atomic Force Microscope for Studying Large Biological Systems

Byung I. Kim; Ryan Boehm

doi:10.1016/j.micron.2012.04.005

Force-Feedback High-Speed Atomic Force Microscope for Studying Large Biological Systems

Byung I. Kim, Ryan Boehm

Physics Department

Boise State University

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

We designed and developed a high-speed atomic force microscope (HSAFM) utilizing a force-feedback scheme for imaging large biological samples. The system collects three simultaneous images: a deflection image, a topographic image, and a force image. We demonstrated that this force-feedback HSAFM is capable of acquiring large topographic images of Escherichia coli biofilms at approximately one frame per second in air. We discuss how the self-actuating cantilever and the piezo tube follow those larger biological topographic features during the HSAFM imaging process.

Original language	American English
Pages (from-to)	1372-1379
Number of pages	8
Journal	Micron
Volume	43
Issue number	12
DOIs	https://doi.org/10.1016/j.micron.2012.04.005
State	Published - Dec 2012

Keywords

Biological AFM
Biological dynamics
COIFM
Force-feedback
High-speed AFM
Interfacial force microscope

EGS Disciplines

Physics

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10.1016/j.micron.2012.04.005

Cite this

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title = "Force-Feedback High-Speed Atomic Force Microscope for Studying Large Biological Systems",

abstract = " We designed and developed a high-speed atomic force microscope (HSAFM) utilizing a force-feedback scheme for imaging large biological samples. The system collects three simultaneous images: a deflection image, a topographic image, and a force image. We demonstrated that this force-feedback HSAFM is capable of acquiring large topographic images of Escherichia coli biofilms at approximately one frame per second in air. We discuss how the self-actuating cantilever and the piezo tube follow those larger biological topographic features during the HSAFM imaging process.",

keywords = "Biological AFM, Biological dynamics, COIFM, Force-feedback, High-speed AFM, Interfacial force microscope",

author = "Kim, {Byung I.} and Ryan Boehm",

year = "2012",

month = dec,

doi = "10.1016/j.micron.2012.04.005",

language = "American English",

volume = "43",

pages = "1372--1379",

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TY - JOUR

T1 - Force-Feedback High-Speed Atomic Force Microscope for Studying Large Biological Systems

AU - Kim, Byung I.

AU - Boehm, Ryan

PY - 2012/12

Y1 - 2012/12

N2 - We designed and developed a high-speed atomic force microscope (HSAFM) utilizing a force-feedback scheme for imaging large biological samples. The system collects three simultaneous images: a deflection image, a topographic image, and a force image. We demonstrated that this force-feedback HSAFM is capable of acquiring large topographic images of Escherichia coli biofilms at approximately one frame per second in air. We discuss how the self-actuating cantilever and the piezo tube follow those larger biological topographic features during the HSAFM imaging process.

AB - We designed and developed a high-speed atomic force microscope (HSAFM) utilizing a force-feedback scheme for imaging large biological samples. The system collects three simultaneous images: a deflection image, a topographic image, and a force image. We demonstrated that this force-feedback HSAFM is capable of acquiring large topographic images of Escherichia coli biofilms at approximately one frame per second in air. We discuss how the self-actuating cantilever and the piezo tube follow those larger biological topographic features during the HSAFM imaging process.

KW - Biological AFM

KW - Biological dynamics

KW - COIFM

KW - Force-feedback

KW - High-speed AFM

KW - Interfacial force microscope

UR - http://www.scopus.com/inward/record.url?scp=84865810551&partnerID=8YFLogxK

UR - https://scholarworks.boisestate.edu/physics_facpubs/113

U2 - 10.1016/j.micron.2012.04.005

DO - 10.1016/j.micron.2012.04.005

M3 - Article

C2 - 22555172

SN - 0968-4328

VL - 43

SP - 1372

EP - 1379

JO - Micron

JF - Micron

IS - 12

ER -

Force-Feedback High-Speed Atomic Force Microscope for Studying Large Biological Systems

Abstract

Keywords

EGS Disciplines

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