Abstract
We designed and developed a high-speed atomic force microscope (HSAFM) utilizing a force-feedback scheme for imaging large biological samples. The system collects three simultaneous images: a deflection image, a topographic image, and a force image. We demonstrated that this force-feedback HSAFM is capable of acquiring large topographic images of Escherichia coli biofilms at approximately one frame per second in air. We discuss how the self-actuating cantilever and the piezo tube follow those larger biological topographic features during the HSAFM imaging process.
| Original language | American English |
|---|---|
| Pages (from-to) | 1372-1379 |
| Number of pages | 8 |
| Journal | Micron |
| Volume | 43 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2012 |
Keywords
- Biological AFM
- Biological dynamics
- COIFM
- Force-feedback
- High-speed AFM
- Interfacial force microscope
EGS Disciplines
- Physics