Gene transfer by electroporation into intact tobacco petiole tissue

Daniel Fologea; Aurelia Brezeanu; Mihai Radu; Petruta Cornea; Ion Vatafu

doi:10.3109/15368379909012893

Gene transfer by electroporation into intact tobacco petiole tissue

Daniel Fologea, Aurelia Brezeanu, Mihai Radu, Petruta Cornea, Ion Vatafu

Physics Department

Research output: Contribution to journal › Article › peer-review

Abstract

Electroporation has been investigated as a potential transformation method for Nicotiana tabacum cv.xanthi intact petiole tissue. Field strength values from 0.25 to 2 kV/cm, one and five exponential pulses, 20 ms width, were used. The experiments were done with a pJIT 79 plasmid, containing β-glucuronidase (gus) and neomycin phosphotransferase (npt II) genes, conferring GUS activity and kanamycin (Km) resistance. Resistant explants were selected in the presence of maximum 100 μg/ml Km. Maximal transformation efficiency of about 50% was observed for one pulse up to 1 kV/cm. A histochemical GUS assay was performed 8 weeks after electroporation and revealed the activity of the introduced gene for different electrical pulse conditions.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Electromagnetic Biology and Medicine
Volume	18
Issue number	1
DOIs	https://doi.org/10.3109/15368379909012893
State	Published - 1999

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title = "Gene transfer by electroporation into intact tobacco petiole tissue",

abstract = "Electroporation has been investigated as a potential transformation method for Nicotiana tabacum cv.xanthi intact petiole tissue. Field strength values from 0.25 to 2 kV/cm, one and five exponential pulses, 20 ms width, were used. The experiments were done with a pJIT 79 plasmid, containing β-glucuronidase (gus) and neomycin phosphotransferase (npt II) genes, conferring GUS activity and kanamycin (Km) resistance. Resistant explants were selected in the presence of maximum 100 μg/ml Km. Maximal transformation efficiency of about 50% was observed for one pulse up to 1 kV/cm. A histochemical GUS assay was performed 8 weeks after electroporation and revealed the activity of the introduced gene for different electrical pulse conditions.",

author = "Daniel Fologea and Aurelia Brezeanu and Mihai Radu and Petruta Cornea and Ion Vatafu",

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T1 - Gene transfer by electroporation into intact tobacco petiole tissue

AU - Fologea, Daniel

AU - Brezeanu, Aurelia

AU - Radu, Mihai

AU - Cornea, Petruta

AU - Vatafu, Ion

PY - 1999

Y1 - 1999

N2 - Electroporation has been investigated as a potential transformation method for Nicotiana tabacum cv.xanthi intact petiole tissue. Field strength values from 0.25 to 2 kV/cm, one and five exponential pulses, 20 ms width, were used. The experiments were done with a pJIT 79 plasmid, containing β-glucuronidase (gus) and neomycin phosphotransferase (npt II) genes, conferring GUS activity and kanamycin (Km) resistance. Resistant explants were selected in the presence of maximum 100 μg/ml Km. Maximal transformation efficiency of about 50% was observed for one pulse up to 1 kV/cm. A histochemical GUS assay was performed 8 weeks after electroporation and revealed the activity of the introduced gene for different electrical pulse conditions.

AB - Electroporation has been investigated as a potential transformation method for Nicotiana tabacum cv.xanthi intact petiole tissue. Field strength values from 0.25 to 2 kV/cm, one and five exponential pulses, 20 ms width, were used. The experiments were done with a pJIT 79 plasmid, containing β-glucuronidase (gus) and neomycin phosphotransferase (npt II) genes, conferring GUS activity and kanamycin (Km) resistance. Resistant explants were selected in the presence of maximum 100 μg/ml Km. Maximal transformation efficiency of about 50% was observed for one pulse up to 1 kV/cm. A histochemical GUS assay was performed 8 weeks after electroporation and revealed the activity of the introduced gene for different electrical pulse conditions.

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Gene transfer by electroporation into intact tobacco petiole tissue

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