Graphene Foam as a Three-Dimensional Platform for Myotube Growth

Eric Krueger; A. Nicole Chang; Dale Brown; Josh Eixenberger; Raquel Brown; Sepideh Rastegar; Katie M. Yocham; Kurtis D. Cantley; David Estrada

doi:10.1021/acsbiomaterials.6b00139

Graphene Foam as a Three-Dimensional Platform for Myotube Growth

Eric Krueger, A. Nicole Chang, Dale Brown, Josh Eixenberger, Raquel Brown, Sepideh Rastegar, Katie M. Yocham, Kurtis D. Cantley, David Estrada

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on three-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin-coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca²⁺ fluorescence, respectively. Further, our data show that the application of a pulsed electrical stimulus to the graphene foam initiates myotube contraction and subsequent localized substrate movement of over 100 μm. These findings will further the development of advanced three-dimensional graphene platforms for therapeutic applications and tissue engineering.

Original language	American English
Pages (from-to)	1234-1241
Number of pages	8
Journal	ACS Biomaterials Science & Engineering
Volume	2
Issue number	8
DOIs	https://doi.org/10.1021/acsbiomaterials.6b00139
State	Published - 8 Aug 2016

Keywords

graphene foam
C2C12
extracellualr matrix
tissue engineering scaffold
confocal microscopy
X-ray micro-CT
extracellular matrix

EGS Disciplines

Materials Science and Engineering

Access to Document

10.1021/acsbiomaterials.6b00139

https://doi.org/10.1021/acsbiomaterials.6b00139

Cite this

@article{f6e770b11fed412283b2ac25c1920d29,

title = "Graphene Foam as a Three-Dimensional Platform for Myotube Growth",

abstract = "This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on three-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin-coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca2+ fluorescence, respectively. Further, our data show that the application of a pulsed electrical stimulus to the graphene foam initiates myotube contraction and subsequent localized substrate movement of over 100 μm. These findings will further the development of advanced three-dimensional graphene platforms for therapeutic applications and tissue engineering.",

keywords = "graphene foam, C2C12, extracellualr matrix, tissue engineering scaffold, confocal microscopy, X-ray micro-CT, extracellular matrix",

author = "Eric Krueger and Chang, {A. Nicole} and Dale Brown and Josh Eixenberger and Raquel Brown and Sepideh Rastegar and Yocham, {Katie M.} and Cantley, {Kurtis D.} and David Estrada",

note = "Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

year = "2016",

month = aug,

day = "8",

doi = "10.1021/acsbiomaterials.6b00139",

language = "American English",

volume = "2",

pages = "1234--1241",

number = "8",

}

TY - JOUR

T1 - Graphene Foam as a Three-Dimensional Platform for Myotube Growth

AU - Krueger, Eric

AU - Chang, A. Nicole

AU - Brown, Dale

AU - Eixenberger, Josh

AU - Brown, Raquel

AU - Rastegar, Sepideh

AU - Yocham, Katie M.

AU - Cantley, Kurtis D.

AU - Estrada, David

PY - 2016/8/8

Y1 - 2016/8/8

N2 - This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on three-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin-coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca2+ fluorescence, respectively. Further, our data show that the application of a pulsed electrical stimulus to the graphene foam initiates myotube contraction and subsequent localized substrate movement of over 100 μm. These findings will further the development of advanced three-dimensional graphene platforms for therapeutic applications and tissue engineering.

AB - This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on three-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin-coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca2+ fluorescence, respectively. Further, our data show that the application of a pulsed electrical stimulus to the graphene foam initiates myotube contraction and subsequent localized substrate movement of over 100 μm. These findings will further the development of advanced three-dimensional graphene platforms for therapeutic applications and tissue engineering.

KW - graphene foam

KW - C2C12

KW - extracellualr matrix

KW - tissue engineering scaffold

KW - confocal microscopy

KW - X-ray micro-CT

KW - extracellular matrix

UR - https://scholarworks.boisestate.edu/mse_facpubs/510

UR - http://www.scopus.com/inward/record.url?scp=85006184844&partnerID=8YFLogxK

U2 - 10.1021/acsbiomaterials.6b00139

DO - 10.1021/acsbiomaterials.6b00139

M3 - Article

VL - 2

SP - 1234

EP - 1241

JO - ACS Biomaterials Science & Engineering

JF - ACS Biomaterials Science & Engineering

IS - 8

ER -

Graphene Foam as a Three-Dimensional Platform for Myotube Growth

Abstract

Keywords

EGS Disciplines

Access to Document

Other files and links

Fingerprint

Cite this