TY - JOUR
T1 - Improved Extraction and Complete Mass Spectral Characterization of Steroidal Alkaloids from Veratrum californicum
AU - Chandler, Christopher M.
AU - Habig, Jeffrey W.
AU - Fisher, Ashley A.
AU - Ambrose, Katherine V.
AU - Jiménez, Susana T.
AU - McDougal, Owen M.
PY - 2013/8
Y1 - 2013/8
N2 - Four steroidal alkaloids extracted from the roots and rhizomes of Veratrum californicum were separated by high performance liquid chromatography (HPLC) and identified using high resolution electrospray ionization time of flight tandem mass spectrometry (ESI-TOF-MS/MS) as veratrosine, cycloposine, veratramine, and cyclopamine. This paper compares ethanol and benzene as extraction solvents, HPLC solvent conditions leading to good separation of steroidal alkaloids, and MS/MS fragmentation patterns for the four steroidal alkaloids which have been released to the public database MassBank.jp. The reported Soxhlet extraction method nearly triples the recovery of steroidal alkaloids from V. californicum.
AB - Four steroidal alkaloids extracted from the roots and rhizomes of Veratrum californicum were separated by high performance liquid chromatography (HPLC) and identified using high resolution electrospray ionization time of flight tandem mass spectrometry (ESI-TOF-MS/MS) as veratrosine, cycloposine, veratramine, and cyclopamine. This paper compares ethanol and benzene as extraction solvents, HPLC solvent conditions leading to good separation of steroidal alkaloids, and MS/MS fragmentation patterns for the four steroidal alkaloids which have been released to the public database MassBank.jp. The reported Soxhlet extraction method nearly triples the recovery of steroidal alkaloids from V. californicum.
KW - Cyclopamine
KW - Mass spectrometry
KW - Steroidal alkaloids
KW - Veratrum californicum
UR - http://www.scopus.com/inward/record.url?scp=84883230831&partnerID=8YFLogxK
UR - https://scholarworks.boisestate.edu/chem_facpubs/8
U2 - 10.1177/1934578x1300800806
DO - 10.1177/1934578x1300800806
M3 - Article
C2 - 24079166
SN - 1934-578X
VL - 8
SP - 1059
EP - 1064
JO - Natural Product Communications
JF - Natural Product Communications
IS - 8
ER -