TY - JOUR
T1 - Increase of Saccharomyces cerevisiae plating efficiency after treatment with bipolar electric pulses
AU - Fologea, D.
AU - Vassu-Dimov, Tatiana
AU - Stoica, Ileana
AU - Csutak, Ortansa
AU - Radu, M.
PY - 1998/10
Y1 - 1998/10
N2 - Survival rate of yeast cells after electroporation with a succession of bipolar pulses was investigated. Field strength values from 0 to 1.5 kV/cm with a 8 ms width of pulses succession were used. After the electric treatment, cells were immediately inoculated in YEPG medium and kept 2 h at 30°C. Then cells were plated on standard solid medium and two days later, platting efficiency was recorded. A maximum of UFC appears around the value of 0.85 kV/cm, the number of the obtained colonies being about 100% higher than the lowest value of the field used. Copyright (C) 1998 Elsevier Science S.A.
AB - Survival rate of yeast cells after electroporation with a succession of bipolar pulses was investigated. Field strength values from 0 to 1.5 kV/cm with a 8 ms width of pulses succession were used. After the electric treatment, cells were immediately inoculated in YEPG medium and kept 2 h at 30°C. Then cells were plated on standard solid medium and two days later, platting efficiency was recorded. A maximum of UFC appears around the value of 0.85 kV/cm, the number of the obtained colonies being about 100% higher than the lowest value of the field used. Copyright (C) 1998 Elsevier Science S.A.
KW - Electric pulses
KW - Electrostimulation
KW - Plating efficiency
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=0032190881&partnerID=8YFLogxK
U2 - 10.1016/S0302-4598(98)00139-1
DO - 10.1016/S0302-4598(98)00139-1
M3 - Article
AN - SCOPUS:0032190881
SN - 0302-4598
VL - 46
SP - 285
EP - 287
JO - Bioelectrochemistry and Bioenergetics
JF - Bioelectrochemistry and Bioenergetics
IS - 2
ER -