Increase of Saccharomyces cerevisiae plating efficiency after treatment with bipolar electric pulses

D. Fologea; Tatiana Vassu-Dimov; Ileana Stoica; Ortansa Csutak; M. Radu

doi:10.1016/S0302-4598(98)00139-1

Increase of Saccharomyces cerevisiae plating efficiency after treatment with bipolar electric pulses

D. Fologea
, Tatiana Vassu-Dimov
, Ileana Stoica
, Ortansa Csutak
, M. Radu

Physics Department

University of Bucharest
Horia Hulubei National Institute of Physics and Nuclear Engineering

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Survival rate of yeast cells after electroporation with a succession of bipolar pulses was investigated. Field strength values from 0 to 1.5 kV/cm with a 8 ms width of pulses succession were used. After the electric treatment, cells were immediately inoculated in YEPG medium and kept 2 h at 30°C. Then cells were plated on standard solid medium and two days later, platting efficiency was recorded. A maximum of UFC appears around the value of 0.85 kV/cm, the number of the obtained colonies being about 100% higher than the lowest value of the field used. Copyright (C) 1998 Elsevier Science S.A.

Original language	English
Pages (from-to)	285-287
Number of pages	3
Journal	Bioelectrochemistry and Bioenergetics
Volume	46
Issue number	2
DOIs	https://doi.org/10.1016/S0302-4598(98)00139-1
State	Published - Oct 1998

Keywords

Electric pulses
Electrostimulation
Plating efficiency
Yeast

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10.1016/S0302-4598(98)00139-1

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title = "Increase of Saccharomyces cerevisiae plating efficiency after treatment with bipolar electric pulses",

abstract = "Survival rate of yeast cells after electroporation with a succession of bipolar pulses was investigated. Field strength values from 0 to 1.5 kV/cm with a 8 ms width of pulses succession were used. After the electric treatment, cells were immediately inoculated in YEPG medium and kept 2 h at 30°C. Then cells were plated on standard solid medium and two days later, platting efficiency was recorded. A maximum of UFC appears around the value of 0.85 kV/cm, the number of the obtained colonies being about 100\% higher than the lowest value of the field used. Copyright (C) 1998 Elsevier Science S.A.",

keywords = "Electric pulses, Electrostimulation, Plating efficiency, Yeast",

author = "D. Fologea and Tatiana Vassu-Dimov and Ileana Stoica and Ortansa Csutak and M. Radu",

year = "1998",

month = oct,

doi = "10.1016/S0302-4598(98)00139-1",

language = "English",

volume = "46",

pages = "285--287",

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TY - JOUR

T1 - Increase of Saccharomyces cerevisiae plating efficiency after treatment with bipolar electric pulses

AU - Fologea, D.

AU - Vassu-Dimov, Tatiana

AU - Stoica, Ileana

AU - Csutak, Ortansa

AU - Radu, M.

PY - 1998/10

Y1 - 1998/10

N2 - Survival rate of yeast cells after electroporation with a succession of bipolar pulses was investigated. Field strength values from 0 to 1.5 kV/cm with a 8 ms width of pulses succession were used. After the electric treatment, cells were immediately inoculated in YEPG medium and kept 2 h at 30°C. Then cells were plated on standard solid medium and two days later, platting efficiency was recorded. A maximum of UFC appears around the value of 0.85 kV/cm, the number of the obtained colonies being about 100% higher than the lowest value of the field used. Copyright (C) 1998 Elsevier Science S.A.

AB - Survival rate of yeast cells after electroporation with a succession of bipolar pulses was investigated. Field strength values from 0 to 1.5 kV/cm with a 8 ms width of pulses succession were used. After the electric treatment, cells were immediately inoculated in YEPG medium and kept 2 h at 30°C. Then cells were plated on standard solid medium and two days later, platting efficiency was recorded. A maximum of UFC appears around the value of 0.85 kV/cm, the number of the obtained colonies being about 100% higher than the lowest value of the field used. Copyright (C) 1998 Elsevier Science S.A.

KW - Electric pulses

KW - Electrostimulation

KW - Plating efficiency

KW - Yeast

UR - https://www.scopus.com/pages/publications/0032190881

U2 - 10.1016/S0302-4598(98)00139-1

DO - 10.1016/S0302-4598(98)00139-1

M3 - Article

AN - SCOPUS:0032190881

SN - 0302-4598

VL - 46

SP - 285

EP - 287

JO - Bioelectrochemistry and Bioenergetics

JF - Bioelectrochemistry and Bioenergetics

IS - 2

ER -

Increase of Saccharomyces cerevisiae plating efficiency after treatment with bipolar electric pulses

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Keywords

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