Abstract
Aptamers are nucleic acid or peptide molecules selected to have a high affinity and specificity for their substrates, very often in the sub-nanomolar range. Consequently, precise and reliable determination of their binding constant to the targets using techniques traditionally based on real-time monitoring is extremely challenging. To alleviate these roadblocks, we investigated the usefulness of the Kinetics Exclusion Assay (KinExA) technology to assess the equilibrium when high-affinity aptamers are employed. Although this technology was specifically developed for characterization of antibody-antigen interactions down to the femtomolar range, we adapted it for routine measurements of interactions that employ DNA aptamers.
| Original language | American English |
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| State | Published - 12 Apr 2019 |