Optimization of Multiplex PCR for Multiple Locus Variable Number Tandem Repeat Analysis for Salmonella typhimurium

George Hafez; Remington Turner; Andrea Haskett; Rajesh Nagarajan

Optimization of Multiplex PCR for Multiple Locus Variable Number Tandem Repeat Analysis for Salmonella typhimurium

George Hafez, Remington Turner, Andrea Haskett, Rajesh Nagarajan

Chemistry Department

Boise State University

Research output: Contribution to conference › Presentation

Abstract

Salmonella typhimurium infects an estimated 1.4 million in the United States and kills over five hundred annually. The current way of categorization, Pulse Field Gel Electrophoresis (PFGE) and antibiotic sensitivity testing, lack the ability to distinguish between some closely related strains. We are attempting to optimize a new technique of categorization, Multiple Locus Variable number tandem repeat Analysis (MLVA). We made changes to temperature and concentration variables within the multiplex PCR procedure to streamline the identification process. By optimizing the multiplex PCR procedure we intend to create more consistent amplification of the loci used in MLVA analysis, which will improve efficiency in differentiating S. typhimurium isolates.

Original language	American English
State	Published - 11 Apr 2011
Event	2011 Undergraduate Research and Scholarship Conference - Boise State University, Boise, United States Duration: 1 Apr 2011 → … https://scholarworks.boisestate.edu/2011_under_conf/

Conference

Conference	2011 Undergraduate Research and Scholarship Conference
Abbreviated title	URS 2011
Country/Territory	United States
City	Boise
Period	1/04/11 → …
Internet address	https://scholarworks.boisestate.edu/2011_under_conf/

EGS Disciplines

Chemistry

Access to Document

https://scholarworks.boisestate.edu/as_11/2

Cite this

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title = "Optimization of Multiplex PCR for Multiple Locus Variable Number Tandem Repeat Analysis for Salmonella typhimurium",

abstract = " Salmonella typhimurium infects an estimated 1.4 million in the United States and kills over five hundred annually. The current way of categorization, Pulse Field Gel Electrophoresis (PFGE) and antibiotic sensitivity testing, lack the ability to distinguish between some closely related strains. We are attempting to optimize a new technique of categorization, Multiple Locus Variable number tandem repeat Analysis (MLVA). We made changes to temperature and concentration variables within the multiplex PCR procedure to streamline the identification process. By optimizing the multiplex PCR procedure we intend to create more consistent amplification of the loci used in MLVA analysis, which will improve efficiency in differentiating S. typhimurium isolates.",

author = "George Hafez and Remington Turner and Andrea Haskett and Rajesh Nagarajan",

year = "2011",

month = apr,

day = "11",

language = "American English",

note = "2011 Undergraduate Research and Scholarship Conference, URS 2011 ; Conference date: 01-04-2011",

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TY - CONF

T1 - Optimization of Multiplex PCR for Multiple Locus Variable Number Tandem Repeat Analysis for Salmonella typhimurium

AU - Hafez, George

AU - Turner, Remington

AU - Haskett, Andrea

AU - Nagarajan, Rajesh

PY - 2011/4/11

Y1 - 2011/4/11

N2 - Salmonella typhimurium infects an estimated 1.4 million in the United States and kills over five hundred annually. The current way of categorization, Pulse Field Gel Electrophoresis (PFGE) and antibiotic sensitivity testing, lack the ability to distinguish between some closely related strains. We are attempting to optimize a new technique of categorization, Multiple Locus Variable number tandem repeat Analysis (MLVA). We made changes to temperature and concentration variables within the multiplex PCR procedure to streamline the identification process. By optimizing the multiplex PCR procedure we intend to create more consistent amplification of the loci used in MLVA analysis, which will improve efficiency in differentiating S. typhimurium isolates.

AB - Salmonella typhimurium infects an estimated 1.4 million in the United States and kills over five hundred annually. The current way of categorization, Pulse Field Gel Electrophoresis (PFGE) and antibiotic sensitivity testing, lack the ability to distinguish between some closely related strains. We are attempting to optimize a new technique of categorization, Multiple Locus Variable number tandem repeat Analysis (MLVA). We made changes to temperature and concentration variables within the multiplex PCR procedure to streamline the identification process. By optimizing the multiplex PCR procedure we intend to create more consistent amplification of the loci used in MLVA analysis, which will improve efficiency in differentiating S. typhimurium isolates.

M3 - Presentation

T2 - 2011 Undergraduate Research and Scholarship Conference

Y2 - 1 April 2011

ER -

Optimization of Multiplex PCR for Multiple Locus Variable Number Tandem Repeat Analysis for Salmonella typhimurium

Abstract

Conference

EGS Disciplines

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