Ovine PIA Arachnoid Complex: Biomechanical Characterization

Gabryel Conley Natividad; Sophia K. Theodossiou; Nathan R. Schiele; Gordon Murdoch; Goutham Burla; Gabriel Potirniche

Ovine PIA Arachnoid Complex: Biomechanical Characterization

Gabryel Conley Natividad, Sophia K. Theodossiou, Nathan R. Schiele, Gordon Murdoch, Goutham Burla, Gabriel Potirniche

University of Idaho

Research output: Contribution to conference › Presentation

Abstract

   Introduction:  The pia arachnoid complex (PAC) is a cerebrospinal fluid-filled tissue that surrounds the brain and spinal cord. Within the PAC, arachnoid trabeculae (AT) fibers, AT sheets, and blood vessels span the space between the arachnoid and pia surfaces. Due to its structural role, alterations to the biomechanical properties of the PAC caused by sub-concussive hits could impact traumatic brain injury (TBI). The aim of this study was to quantify the mechanical and structural properties of ovine PAC.
   Methods:  Ovine brain samples (n = 10) were harvested and removed from the skull within 30 min post-mortem. To access the brain tissue, skulls were split medially from the occipital region to down to the nose on the superior and inferior aspects of the skull. A template was used to remove brain samples from different regions of the brain. At < 2-h PAC samples were tested with uniaxial tension at ~ 2 mm/s until failure. The force and displacement data were acquired at 100 Hz using LabVIEW and tissue structure was characterized using confocal microscopy.
   Results:  An average strain rate of 0.59 s−1 was observed for PAC samples subjected to uniaxial tension. Using a Mooney-Rivlin model for average stress-strain curve fit, Young’s modulus of the linear region was found to be 7.68 ± 3.0 MPa. The mean ultimate stress and strain were found to be 2.69 ± 0.76 MPa and 0.60 ± 0.13, respectively. There were no significant differences across sample locations.
   Conclusions:  To our knowledge, this preliminary study represents the first biomechanical characterization of ovine PAC.

Original language	American English
State	Published - Sep 2019
Externally published	Yes
Event	11th Meeting of the International Society for Hydrocephalus and Cerebrospinal Fluid Disorders - Vancouver, Canada Duration: 1 Sep 2019 → …

Conference

Conference	11th Meeting of the International Society for Hydrocephalus and Cerebrospinal Fluid Disorders
Period	1/09/19 → …

EGS Disciplines

Biomedical Engineering and Bioengineering
Mechanical Engineering

Cite this

@conference{5950d232920546928786042ff5938cb9,

title = "Ovine PIA Arachnoid Complex: Biomechanical Characterization",

abstract = " Introduction: The pia arachnoid complex (PAC) is a cerebrospinal fluid-filled tissue that surrounds the brain and spinal cord. Within the PAC, arachnoid trabeculae (AT) fibers, AT sheets, and blood vessels span the space between the arachnoid and pia surfaces. Due to its structural role, alterations to the biomechanical properties of the PAC caused by sub-concussive hits could impact traumatic brain injury (TBI). The aim of this study was to quantify the mechanical and structural properties of ovine PAC. Methods: Ovine brain samples (n = 10) were harvested and removed from the skull within 30 min post-mortem. To access the brain tissue, skulls were split medially from the occipital region to down to the nose on the superior and inferior aspects of the skull. A template was used to remove brain samples from different regions of the brain. At < 2-h PAC samples were tested with uniaxial tension at ~ 2 mm/s until failure. The force and displacement data were acquired at 100 Hz using LabVIEW and tissue structure was characterized using confocal microscopy. Results: An average strain rate of 0.59 s−1 was observed for PAC samples subjected to uniaxial tension. Using a Mooney-Rivlin model for average stress-strain curve fit, Young{\textquoteright}s modulus of the linear region was found to be 7.68 ± 3.0 MPa. The mean ultimate stress and strain were found to be 2.69 ± 0.76 MPa and 0.60 ± 0.13, respectively. There were no significant differences across sample locations. Conclusions: To our knowledge, this preliminary study represents the first biomechanical characterization of ovine PAC.",

author = "Natividad, {Gabryel Conley} and Theodossiou, {Sophia K.} and Schiele, {Nathan R.} and Gordon Murdoch and Goutham Burla and Gabriel Potirniche",

note = "Conley Natividad, Gabryel; Theodossiou, Sophia K.; Schiele, Nathan R.; Murdoch, Gordon; Burla, Goutham; Potirniche, Gabriel; . . . and Martin, Bryn A. (2019). {"}Ovine PIA Arachnoid Complex: Biomechanical Characterization{"}. 11th Meeting of the International Society for Hydrocephalus and Cerebrospinal Fluid Disorders.; 11th Meeting of the International Society for Hydrocephalus and Cerebrospinal Fluid Disorders ; Conference date: 01-09-2019",

year = "2019",

month = sep,

language = "American English",

}

TY - CONF

T1 - Ovine PIA Arachnoid Complex: Biomechanical Characterization

AU - Natividad, Gabryel Conley

AU - Theodossiou, Sophia K.

AU - Schiele, Nathan R.

AU - Murdoch, Gordon

AU - Burla, Goutham

AU - Potirniche, Gabriel

N1 - Conley Natividad, Gabryel; Theodossiou, Sophia K.; Schiele, Nathan R.; Murdoch, Gordon; Burla, Goutham; Potirniche, Gabriel; . . . and Martin, Bryn A. (2019). "Ovine PIA Arachnoid Complex: Biomechanical Characterization". 11th Meeting of the International Society for Hydrocephalus and Cerebrospinal Fluid Disorders.

PY - 2019/9

Y1 - 2019/9

N2 - Introduction: The pia arachnoid complex (PAC) is a cerebrospinal fluid-filled tissue that surrounds the brain and spinal cord. Within the PAC, arachnoid trabeculae (AT) fibers, AT sheets, and blood vessels span the space between the arachnoid and pia surfaces. Due to its structural role, alterations to the biomechanical properties of the PAC caused by sub-concussive hits could impact traumatic brain injury (TBI). The aim of this study was to quantify the mechanical and structural properties of ovine PAC. Methods: Ovine brain samples (n = 10) were harvested and removed from the skull within 30 min post-mortem. To access the brain tissue, skulls were split medially from the occipital region to down to the nose on the superior and inferior aspects of the skull. A template was used to remove brain samples from different regions of the brain. At < 2-h PAC samples were tested with uniaxial tension at ~ 2 mm/s until failure. The force and displacement data were acquired at 100 Hz using LabVIEW and tissue structure was characterized using confocal microscopy. Results: An average strain rate of 0.59 s−1 was observed for PAC samples subjected to uniaxial tension. Using a Mooney-Rivlin model for average stress-strain curve fit, Young’s modulus of the linear region was found to be 7.68 ± 3.0 MPa. The mean ultimate stress and strain were found to be 2.69 ± 0.76 MPa and 0.60 ± 0.13, respectively. There were no significant differences across sample locations. Conclusions: To our knowledge, this preliminary study represents the first biomechanical characterization of ovine PAC.

AB - Introduction: The pia arachnoid complex (PAC) is a cerebrospinal fluid-filled tissue that surrounds the brain and spinal cord. Within the PAC, arachnoid trabeculae (AT) fibers, AT sheets, and blood vessels span the space between the arachnoid and pia surfaces. Due to its structural role, alterations to the biomechanical properties of the PAC caused by sub-concussive hits could impact traumatic brain injury (TBI). The aim of this study was to quantify the mechanical and structural properties of ovine PAC. Methods: Ovine brain samples (n = 10) were harvested and removed from the skull within 30 min post-mortem. To access the brain tissue, skulls were split medially from the occipital region to down to the nose on the superior and inferior aspects of the skull. A template was used to remove brain samples from different regions of the brain. At < 2-h PAC samples were tested with uniaxial tension at ~ 2 mm/s until failure. The force and displacement data were acquired at 100 Hz using LabVIEW and tissue structure was characterized using confocal microscopy. Results: An average strain rate of 0.59 s−1 was observed for PAC samples subjected to uniaxial tension. Using a Mooney-Rivlin model for average stress-strain curve fit, Young’s modulus of the linear region was found to be 7.68 ± 3.0 MPa. The mean ultimate stress and strain were found to be 2.69 ± 0.76 MPa and 0.60 ± 0.13, respectively. There were no significant differences across sample locations. Conclusions: To our knowledge, this preliminary study represents the first biomechanical characterization of ovine PAC.

M3 - Presentation

T2 - 11th Meeting of the International Society for Hydrocephalus and Cerebrospinal Fluid Disorders

Y2 - 1 September 2019

ER -

Ovine PIA Arachnoid Complex: Biomechanical Characterization

Abstract

Conference

EGS Disciplines

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