Printable Bioscaffolds Using MXenes for Musculoskeletal Tissue Engineering

Annaka Tibbits; David Estrada; Hailey Burgoyne; Fereshteh Rajabi-Kouchi; Raquel Montenegro-Brown; Miranda L. Nelson

Printable Bioscaffolds Using MXenes for Musculoskeletal Tissue Engineering

Annaka Tibbits
, David Estrada
, Hailey Burgoyne
, Fereshteh Rajabi-Kouchi
, Raquel Montenegro-Brown
, Miranda L. Nelson

Micron School of Materials Science and Engineering

Research output: Contribution to conference › Poster

Abstract

Musculoskeletal disease is the number one cause of physical disability in the world. A potential cure for this is tissue engineering; specifically, culturing STEM cells into scaffolds to create tissue specific grafts to be surgically implanted. We successfully printed bioscaffolds with high structural integrity and sufficient conductivity to aid in the functionality of the muscle cells by using cell-laden bioinks with Ti ₃ C ₂ MXene nanoparticles. Furthermore, we tested GelMA, GelXA _, and Cellink bioinks at a seeding density of 1x10 ⁶ cells/mL with 0, 0.1, and 1.0 mg/mL MXene concentrations. UV Crosslinking of GelMA and GelXA was determined to be less functional with higher concentrations of MXenes due to light deflection. Additionally, chemical crosslinking in GelXA and Cellink led to better structural integrity. Overall, MXenes improved the mechanical and electrical properties of the scaffold while maintaining cell viability.

Original language	American English
State	Published - 1 Jul 2023
Event	Idaho Conference on Undergraduate Research 2023 - Boise State University, Boise, United States Duration: 1 Jul 2023 → … https://scholarworks.boisestate.edu/icur/2023/

Conference

Conference	Idaho Conference on Undergraduate Research 2023
Abbreviated title	ICUR 2023
Country/Territory	United States
City	Boise
Period	1/07/23 → …
Internet address	https://scholarworks.boisestate.edu/icur/2023/

Cite this

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title = "Printable Bioscaffolds Using MXenes for Musculoskeletal Tissue Engineering",

abstract = " Musculoskeletal disease is the number one cause of physical disability in the world. A potential cure for this is tissue engineering; specifically, culturing STEM cells into scaffolds to create tissue specific grafts to be surgically implanted. We successfully printed bioscaffolds with high structural integrity and sufficient conductivity to aid in the functionality of the muscle cells by using cell-laden bioinks with Ti 3 C 2 MXene nanoparticles. Furthermore, we tested GelMA, GelXA , and Cellink bioinks at a seeding density of 1x10 6 cells/mL with 0, 0.1, and 1.0 mg/mL MXene concentrations. UV Crosslinking of GelMA and GelXA was determined to be less functional with higher concentrations of MXenes due to light deflection. Additionally, chemical crosslinking in GelXA and Cellink led to better structural integrity. Overall, MXenes improved the mechanical and electrical properties of the scaffold while maintaining cell viability.",

author = "Annaka Tibbits and David Estrada and Hailey Burgoyne and Fereshteh Rajabi-Kouchi and Raquel Montenegro-Brown and Nelson, \{Miranda L.\}",

year = "2023",

month = jul,

day = "1",

language = "American English",

note = "Idaho Conference on Undergraduate Research 2023, ICUR 2023 ; Conference date: 01-07-2023",

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TY - CONF

T1 - Printable Bioscaffolds Using MXenes for Musculoskeletal Tissue Engineering

AU - Tibbits, Annaka

AU - Estrada, David

AU - Burgoyne, Hailey

AU - Rajabi-Kouchi, Fereshteh

AU - Montenegro-Brown, Raquel

AU - Nelson, Miranda L.

PY - 2023/7/1

Y1 - 2023/7/1

N2 - Musculoskeletal disease is the number one cause of physical disability in the world. A potential cure for this is tissue engineering; specifically, culturing STEM cells into scaffolds to create tissue specific grafts to be surgically implanted. We successfully printed bioscaffolds with high structural integrity and sufficient conductivity to aid in the functionality of the muscle cells by using cell-laden bioinks with Ti 3 C 2 MXene nanoparticles. Furthermore, we tested GelMA, GelXA , and Cellink bioinks at a seeding density of 1x10 6 cells/mL with 0, 0.1, and 1.0 mg/mL MXene concentrations. UV Crosslinking of GelMA and GelXA was determined to be less functional with higher concentrations of MXenes due to light deflection. Additionally, chemical crosslinking in GelXA and Cellink led to better structural integrity. Overall, MXenes improved the mechanical and electrical properties of the scaffold while maintaining cell viability.

AB - Musculoskeletal disease is the number one cause of physical disability in the world. A potential cure for this is tissue engineering; specifically, culturing STEM cells into scaffolds to create tissue specific grafts to be surgically implanted. We successfully printed bioscaffolds with high structural integrity and sufficient conductivity to aid in the functionality of the muscle cells by using cell-laden bioinks with Ti 3 C 2 MXene nanoparticles. Furthermore, we tested GelMA, GelXA , and Cellink bioinks at a seeding density of 1x10 6 cells/mL with 0, 0.1, and 1.0 mg/mL MXene concentrations. UV Crosslinking of GelMA and GelXA was determined to be less functional with higher concentrations of MXenes due to light deflection. Additionally, chemical crosslinking in GelXA and Cellink led to better structural integrity. Overall, MXenes improved the mechanical and electrical properties of the scaffold while maintaining cell viability.

UR - https://scholarworks.boisestate.edu/icur/2023/poster_session/22/

M3 - Poster

T2 - Idaho Conference on Undergraduate Research 2023

Y2 - 1 July 2023

ER -

Printable Bioscaffolds Using MXenes for Musculoskeletal Tissue Engineering

Abstract

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