Programmed adsorption and release of proteins in a microfluidic device

Dale L. Huber, Ronald P. Manginell, Michael A. Samara, Byung Il Kim, Bruce C. Bunker

Research output: Contribution to journalArticlepeer-review

500 Scopus citations

Abstract

A microfluidic device has been developed that can adsorb proteins from solution, hold them with negligible denaturation, and release them on command. The active element in the device is a 4-nanometer-thick polymer film that can be thermally switched between an antifouling hydrophilic state and a protein-adsorbing state that is more hydrophobic. This active polymer has been integrated into a microfluidic hot plate that can be programmed to adsorb and desorb protein monolayers in less than 1 second. The rapid response characteristics of the device can be manipulated for proteomic functions, including preconcentration and separation of soluble proteins on an integrated fluidics chip.

Original languageEnglish
Pages (from-to)352-354
Number of pages3
JournalScience
Volume301
Issue number5631
DOIs
StatePublished - 18 Jul 2003

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