Abstract
A microfluidic device has been developed that can adsorb proteins from solution, hold them with negligible denaturation, and release them on command. The active element in the device is a 4-nanometer-thick polymer film that can be thermally switched between an antifouling hydrophilic state and a protein-adsorbing state that is more hydrophobic. This active polymer has been integrated into a microfluidic hot plate that can be programmed to adsorb and desorb protein monolayers in less than 1 second. The rapid response characteristics of the device can be manipulated for proteomic functions, including preconcentration and separation of soluble proteins on an integrated fluidics chip.
Original language | English |
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Pages (from-to) | 352-354 |
Number of pages | 3 |
Journal | Science |
Volume | 301 |
Issue number | 5631 |
DOIs | |
State | Published - 18 Jul 2003 |