Protection of interferon-γ knockout mice against Listeria monocytogenes challenge following intramuscular immunization with DNA vaccines encoding listeriolysin O

Ronald A. Barry, H. G.Archie Bouwer, Tina R. Clark, Kenneth A. Cornell, David J. Hinrichs

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

In this study we evaluated the efficacy of DNA vaccination of IFN-γ knockout (GKO) mice against Listeria monocytogenes, as these immunodeficient mice are highly susceptible to infection with low numbers of this intracellular bacterial pathogen. Following intramuscular immunization of BALB/c GKO mice with plasmid DNA constructs encoding recombinant forms of the L. monocytogenes hemolysin, listeriolysin O (LLO), we detected the in vivo induction of a LLO91-99 peptide-specific, protective immune CTL response equivalent to that observed following similar DNA vaccination of normal BALB/c mice. The observed protection represented greatly enhanced immunity for the GKO host, suggesting that DNA vaccination may provide a useful vaccine alternative for certain immunocompromised host populations.

Original languageEnglish
Pages (from-to)2122-2132
Number of pages11
JournalVaccine
Volume21
Issue number17-18
DOIs
StatePublished - 16 May 2003

Keywords

  • Bacterial immunity
  • Cytotoxic T lymphocytes
  • DNA vaccines

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