TY - JOUR
T1 - Pulsed and parallel-polarization EPR characterization of the photosystem II oxygen-evolving complex
AU - Britt, R. David
AU - Peloquin, Jeffrey M.
AU - Campbell, Kristy A.
PY - 2000
Y1 - 2000
N2 - Photosystem II uses visible light to drive the oxidation of water, resulting in bioactivated electrons and protons, with the production of molecular oxygen as a byproduct. This water-splitting reaction is carried out by a manganese cluster/tyrosine radical ensemble, the oxygen-evolving complex. Although conventional continuous-wave, perpendicular-polarization electron paramagnetic resonance (EPR) spectroscopy has significantly advanced our knowledge of the structure and function of the oxygen-evolving complex, significant additional information can be obtained with the application of additional EPR methodologies. Specifically, parallel-polarization EPR spectroscopy can be used to obtain highly resolved EPR spectra of integer spin Mn species, and pulsed EPR spectroscopy with electron spin echo-based sequences, such as electron spin echo envelope modulation and electron spin echo-electron nuclear double resonance, can be tised to measure weak interactions obscured in continuous-wave spectroscopy by inhomogeneous broadening.
AB - Photosystem II uses visible light to drive the oxidation of water, resulting in bioactivated electrons and protons, with the production of molecular oxygen as a byproduct. This water-splitting reaction is carried out by a manganese cluster/tyrosine radical ensemble, the oxygen-evolving complex. Although conventional continuous-wave, perpendicular-polarization electron paramagnetic resonance (EPR) spectroscopy has significantly advanced our knowledge of the structure and function of the oxygen-evolving complex, significant additional information can be obtained with the application of additional EPR methodologies. Specifically, parallel-polarization EPR spectroscopy can be used to obtain highly resolved EPR spectra of integer spin Mn species, and pulsed EPR spectroscopy with electron spin echo-based sequences, such as electron spin echo envelope modulation and electron spin echo-electron nuclear double resonance, can be tised to measure weak interactions obscured in continuous-wave spectroscopy by inhomogeneous broadening.
KW - ENDOR
KW - Manganese cluster
KW - Oxygen evolution
KW - Photoactivation
UR - http://www.scopus.com/inward/record.url?scp=0033874086&partnerID=8YFLogxK
U2 - 10.1146/annurev.biophys.29.1.463
DO - 10.1146/annurev.biophys.29.1.463
M3 - Review article
C2 - 10940256
AN - SCOPUS:0033874086
SN - 1056-8700
VL - 29
SP - 463
EP - 495
JO - Annual Review of Biophysics and Biomolecular Structure
JF - Annual Review of Biophysics and Biomolecular Structure
ER -