Pulsed and parallel-polarization EPR characterization of the photosystem II oxygen-evolving complex

R. David Britt, Jeffrey M. Peloquin, Kristy A. Campbell

Research output: Contribution to journalReview articlepeer-review

108 Scopus citations

Abstract

Photosystem II uses visible light to drive the oxidation of water, resulting in bioactivated electrons and protons, with the production of molecular oxygen as a byproduct. This water-splitting reaction is carried out by a manganese cluster/tyrosine radical ensemble, the oxygen-evolving complex. Although conventional continuous-wave, perpendicular-polarization electron paramagnetic resonance (EPR) spectroscopy has significantly advanced our knowledge of the structure and function of the oxygen-evolving complex, significant additional information can be obtained with the application of additional EPR methodologies. Specifically, parallel-polarization EPR spectroscopy can be used to obtain highly resolved EPR spectra of integer spin Mn species, and pulsed EPR spectroscopy with electron spin echo-based sequences, such as electron spin echo envelope modulation and electron spin echo-electron nuclear double resonance, can be tised to measure weak interactions obscured in continuous-wave spectroscopy by inhomogeneous broadening.

Original languageEnglish
Pages (from-to)463-495
Number of pages33
JournalAnnual Review of Biophysics and Biomolecular Structure
Volume29
DOIs
StatePublished - 2000

Keywords

  • ENDOR
  • Manganese cluster
  • Oxygen evolution
  • Photoactivation

Fingerprint

Dive into the research topics of 'Pulsed and parallel-polarization EPR characterization of the photosystem II oxygen-evolving complex'. Together they form a unique fingerprint.

Cite this