Quantifying colorimetric assays in paper-based microfluidic devices by measuring the transmission of light through paper

Audrey K. Ellerbee; Scott T. Phillips; Adam C. Siegel; Katherine A. Mirica; Andres W. Martinez; Pierre Striehl; Nina Jain; Mara Prentiss; George M. Whitesides

doi:10.1021/ac901307q

Quantifying colorimetric assays in paper-based microfluidic devices by measuring the transmission of light through paper

Audrey K. Ellerbee, Scott T. Phillips, Adam C. Siegel, Katherine A. Mirica, Andres W. Martinez, Pierre Striehl, Nina Jain, Mara Prentiss, George M. Whitesides

Micron School of Materials Science and Engineering

Harvard University

Research output: Contribution to journal › Article › peer-review

356 Scopus citations

Abstract

This article describes a point-of-care (POC) system - comprising a microfluidic, paper-based analytical device (μ-PAD) and a hand-held optical colorimeter - for quantifying the concentration of analytes in biological fluids. The μ-PAD runs colorimetric assays, and consists of paper that has been (i) patterned to expose isolated regions of hydrophilic zones and (ii) wet with an index-matching fluid (e.g., vegetable oil) that is applied using a disposable, plastic sleeve encasement. Measuring transmittance through paper represents a new method of quantitative detection that expands the potential functionality of μ-PADs. This prototype transmittance colorimeter is inexpensive, rugged, and fully self-contained, and thus potentially attractive for use in resource-limited environments and developing countries.

Original language	English
Pages (from-to)	8447-8452
Number of pages	6
Journal	Analytical Chemistry
Volume	81
Issue number	20
DOIs	https://doi.org/10.1021/ac901307q
State	Published - 15 Oct 2009

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title = "Quantifying colorimetric assays in paper-based microfluidic devices by measuring the transmission of light through paper",

abstract = "This article describes a point-of-care (POC) system - comprising a microfluidic, paper-based analytical device (μ-PAD) and a hand-held optical colorimeter - for quantifying the concentration of analytes in biological fluids. The μ-PAD runs colorimetric assays, and consists of paper that has been (i) patterned to expose isolated regions of hydrophilic zones and (ii) wet with an index-matching fluid (e.g., vegetable oil) that is applied using a disposable, plastic sleeve encasement. Measuring transmittance through paper represents a new method of quantitative detection that expands the potential functionality of μ-PADs. This prototype transmittance colorimeter is inexpensive, rugged, and fully self-contained, and thus potentially attractive for use in resource-limited environments and developing countries.",

author = "Ellerbee, {Audrey K.} and Phillips, {Scott T.} and Siegel, {Adam C.} and Mirica, {Katherine A.} and Martinez, {Andres W.} and Pierre Striehl and Nina Jain and Mara Prentiss and Whitesides, {George M.}",

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AU - Ellerbee, Audrey K.

AU - Phillips, Scott T.

AU - Siegel, Adam C.

AU - Mirica, Katherine A.

AU - Martinez, Andres W.

AU - Striehl, Pierre

AU - Jain, Nina

AU - Prentiss, Mara

AU - Whitesides, George M.

PY - 2009/10/15

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AB - This article describes a point-of-care (POC) system - comprising a microfluidic, paper-based analytical device (μ-PAD) and a hand-held optical colorimeter - for quantifying the concentration of analytes in biological fluids. The μ-PAD runs colorimetric assays, and consists of paper that has been (i) patterned to expose isolated regions of hydrophilic zones and (ii) wet with an index-matching fluid (e.g., vegetable oil) that is applied using a disposable, plastic sleeve encasement. Measuring transmittance through paper represents a new method of quantitative detection that expands the potential functionality of μ-PADs. This prototype transmittance colorimeter is inexpensive, rugged, and fully self-contained, and thus potentially attractive for use in resource-limited environments and developing countries.

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Quantifying colorimetric assays in paper-based microfluidic devices by measuring the transmission of light through paper

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