TY - JOUR
T1 - Quantifying colorimetric assays in paper-based microfluidic devices by measuring the transmission of light through paper
AU - Ellerbee, Audrey K.
AU - Phillips, Scott T.
AU - Siegel, Adam C.
AU - Mirica, Katherine A.
AU - Martinez, Andres W.
AU - Striehl, Pierre
AU - Jain, Nina
AU - Prentiss, Mara
AU - Whitesides, George M.
PY - 2009/10/15
Y1 - 2009/10/15
N2 - This article describes a point-of-care (POC) system - comprising a microfluidic, paper-based analytical device (μ-PAD) and a hand-held optical colorimeter - for quantifying the concentration of analytes in biological fluids. The μ-PAD runs colorimetric assays, and consists of paper that has been (i) patterned to expose isolated regions of hydrophilic zones and (ii) wet with an index-matching fluid (e.g., vegetable oil) that is applied using a disposable, plastic sleeve encasement. Measuring transmittance through paper represents a new method of quantitative detection that expands the potential functionality of μ-PADs. This prototype transmittance colorimeter is inexpensive, rugged, and fully self-contained, and thus potentially attractive for use in resource-limited environments and developing countries.
AB - This article describes a point-of-care (POC) system - comprising a microfluidic, paper-based analytical device (μ-PAD) and a hand-held optical colorimeter - for quantifying the concentration of analytes in biological fluids. The μ-PAD runs colorimetric assays, and consists of paper that has been (i) patterned to expose isolated regions of hydrophilic zones and (ii) wet with an index-matching fluid (e.g., vegetable oil) that is applied using a disposable, plastic sleeve encasement. Measuring transmittance through paper represents a new method of quantitative detection that expands the potential functionality of μ-PADs. This prototype transmittance colorimeter is inexpensive, rugged, and fully self-contained, and thus potentially attractive for use in resource-limited environments and developing countries.
KW - Animals
KW - Cattle
KW - Clinical Chemistry Tests/instrumentation
KW - Colorimetry/economics
KW - Humans
KW - Light
KW - Microfluidic Analytical Techniques/instrumentation
KW - Paper
KW - Point-of-Care Systems
KW - Proteins/analysis
UR - https://www.scopus.com/pages/publications/70449922272
U2 - 10.1021/ac901307q
DO - 10.1021/ac901307q
M3 - Article
C2 - 19722495
AN - SCOPUS:70449922272
SN - 0003-2700
VL - 81
SP - 8447
EP - 8452
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 20
ER -