Slowing DNA translocation in a solid-state nanopore

Daniel Fologea, James Uplinger, Brian Thomas, David S. McNabb, Jiali Li

Research output: Contribution to journalArticlepeer-review

470 Scopus citations

Abstract

Reducing a DNA molecule's translocation speed in a solid-state nanopore is a key step toward rapid single molecule identification. Here we demonstrate that DNA translocation speeds can be reduced by an order of magnitude over previous results. By controlling the electrolyte temperature, salt concentration, viscosity, and the electrical bias voltage across the nanopore, we obtain a 3 base/μs translocation speed for 3 kbp double-stranded DNA in a 4-8 nm diameter silicon nitride pore. Our results also indicate that the ionic conductivity inside such a nanopore is smaller than it is in bulk.

Original languageEnglish
Pages (from-to)1734-1737
Number of pages4
JournalNano Letters
Volume5
Issue number9
DOIs
StatePublished - Sep 2005

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