1H, 13C and 15N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein

Srdan Matosin; Patrick D. Fischer; Maxim A. Droemer; Eric Baggs; Abu Sayeed Chowdhury; Isidoro Tavares; Scott B. Ficarro; Lisa Rose Warner; Haribabu Arthanari; Rajesh Nagarajan

doi:10.1007/s12104-023-10136-4

¹H, ¹³C and ¹⁵N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein

Srdan Matosin, Patrick D. Fischer, Maxim A. Droemer, Eric Baggs, Abu Sayeed Chowdhury, Isidoro Tavares, Scott B. Ficarro, Lisa Rose Warner, Haribabu Arthanari, Rajesh Nagarajan

Chemistry Department

Research output: Contribution to journal › Article › peer-review

Abstract

Acyl carrier proteins (ACPs) are universally conserved proteins amongst different species and are involved in fatty acid synthesis. Bacteria utilize ACPs as acyl carriers and donors for the synthesis of products such as endotoxins or acyl homoserine lactones (AHLs), which are used in quorum sensing mechanisms. In this study, wehave expressed isotopically labeled holo-ACP from Burkholderia mallei in Escherichia coli to assign 100% of non-proline backbone amide (HN) resonances, 95.5% of aliphatic carbon resonances and 98.6% of aliphatic hydrogen sidechain resonances.

Original language	English
Pages (from-to)	167-171
Number of pages	5
Journal	Biomolecular NMR Assignments
Volume	17
Issue number	2
DOIs	https://doi.org/10.1007/s12104-023-10136-4
State	Published - Dec 2023

Keywords

Acyl carrier proteins
NMR assignment
Quorum sensing

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10.1007/s12104-023-10136-4

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title = "1H, 13C and 15N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein",

abstract = "Acyl carrier proteins (ACPs) are universally conserved proteins amongst different species and are involved in fatty acid synthesis. Bacteria utilize ACPs as acyl carriers and donors for the synthesis of products such as endotoxins or acyl homoserine lactones (AHLs), which are used in quorum sensing mechanisms. In this study, wehave expressed isotopically labeled holo-ACP from Burkholderia mallei in Escherichia coli to assign 100% of non-proline backbone amide (HN) resonances, 95.5% of aliphatic carbon resonances and 98.6% of aliphatic hydrogen sidechain resonances.",

keywords = "Acyl carrier proteins, NMR assignment, Quorum sensing",

author = "Srdan Matosin and Fischer, {Patrick D.} and Droemer, {Maxim A.} and Eric Baggs and Chowdhury, {Abu Sayeed} and Isidoro Tavares and Ficarro, {Scott B.} and Warner, {Lisa Rose} and Haribabu Arthanari and Rajesh Nagarajan",

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doi = "10.1007/s12104-023-10136-4",

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T1 - 1H, 13C and 15N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein

AU - Matosin, Srdan

AU - Fischer, Patrick D.

AU - Droemer, Maxim A.

AU - Baggs, Eric

AU - Chowdhury, Abu Sayeed

AU - Tavares, Isidoro

AU - Ficarro, Scott B.

AU - Warner, Lisa Rose

AU - Arthanari, Haribabu

AU - Nagarajan, Rajesh

PY - 2023/12

Y1 - 2023/12

N2 - Acyl carrier proteins (ACPs) are universally conserved proteins amongst different species and are involved in fatty acid synthesis. Bacteria utilize ACPs as acyl carriers and donors for the synthesis of products such as endotoxins or acyl homoserine lactones (AHLs), which are used in quorum sensing mechanisms. In this study, wehave expressed isotopically labeled holo-ACP from Burkholderia mallei in Escherichia coli to assign 100% of non-proline backbone amide (HN) resonances, 95.5% of aliphatic carbon resonances and 98.6% of aliphatic hydrogen sidechain resonances.

AB - Acyl carrier proteins (ACPs) are universally conserved proteins amongst different species and are involved in fatty acid synthesis. Bacteria utilize ACPs as acyl carriers and donors for the synthesis of products such as endotoxins or acyl homoserine lactones (AHLs), which are used in quorum sensing mechanisms. In this study, wehave expressed isotopically labeled holo-ACP from Burkholderia mallei in Escherichia coli to assign 100% of non-proline backbone amide (HN) resonances, 95.5% of aliphatic carbon resonances and 98.6% of aliphatic hydrogen sidechain resonances.

KW - Acyl carrier proteins

KW - NMR assignment

KW - Quorum sensing

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M3 - Article

C2 - 37233945

AN - SCOPUS:85160333173

SN - 1874-2718

VL - 17

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JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

IS - 2

ER -

¹H, ¹³C and ¹⁵N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein

Abstract

Keywords

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