The migration of dna into a DNA-crosslinked gel using electrophoresis

David C. Lin; Noshir A. Langrana; Bernard Yurke

doi:10.1115/IMECE2003-43446

The migration of dna into a DNA-crosslinked gel using electrophoresis

David C. Lin, Noshir A. Langrana, Bernard Yurke

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

DNA-crosslinked polyacrylamide gels are polymeric electrolytes by virtue of the fact that DNA is negatively charged in an aqueous solution. As such, their mechanical properties can be altered by electrophoretic and electro-osmotic effects. Hybridization of single-stranded DNA with single-stranded sections of the crosslinks provides a novel means of altering gel mechanical properties. As a step toward exploring this means of altering gel mechanical properties, we report here on a study of the use of electrophoresis to introduce single stranded DNA into DNA crosslinked gels. Changes in elastic properties of the gel, before and after electrophoresis, were measured.

Original language	English
Pages (from-to)	131-132
Number of pages	2
Journal	American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Volume	55
DOIs	https://doi.org/10.1115/IMECE2003-43446
State	Published - 2003
Externally published	Yes
Event	2003 ASME International Mechanical Engineering Congress - Washington, DC., United States Duration: 15 Nov 2003 → 21 Nov 2003

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title = "The migration of dna into a DNA-crosslinked gel using electrophoresis",

abstract = "DNA-crosslinked polyacrylamide gels are polymeric electrolytes by virtue of the fact that DNA is negatively charged in an aqueous solution. As such, their mechanical properties can be altered by electrophoretic and electro-osmotic effects. Hybridization of single-stranded DNA with single-stranded sections of the crosslinks provides a novel means of altering gel mechanical properties. As a step toward exploring this means of altering gel mechanical properties, we report here on a study of the use of electrophoresis to introduce single stranded DNA into DNA crosslinked gels. Changes in elastic properties of the gel, before and after electrophoresis, were measured.",

author = "Lin, {David C.} and Langrana, {Noshir A.} and Bernard Yurke",

year = "2003",

doi = "10.1115/IMECE2003-43446",

language = "English",

volume = "55",

pages = "131--132",

journal = "American Society of Mechanical Engineers, Bioengineering Division (Publication) BED",

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note = "2003 ASME International Mechanical Engineering Congress ; Conference date: 15-11-2003 Through 21-11-2003",

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AU - Lin, David C.

AU - Langrana, Noshir A.

AU - Yurke, Bernard

PY - 2003

Y1 - 2003

N2 - DNA-crosslinked polyacrylamide gels are polymeric electrolytes by virtue of the fact that DNA is negatively charged in an aqueous solution. As such, their mechanical properties can be altered by electrophoretic and electro-osmotic effects. Hybridization of single-stranded DNA with single-stranded sections of the crosslinks provides a novel means of altering gel mechanical properties. As a step toward exploring this means of altering gel mechanical properties, we report here on a study of the use of electrophoresis to introduce single stranded DNA into DNA crosslinked gels. Changes in elastic properties of the gel, before and after electrophoresis, were measured.

AB - DNA-crosslinked polyacrylamide gels are polymeric electrolytes by virtue of the fact that DNA is negatively charged in an aqueous solution. As such, their mechanical properties can be altered by electrophoretic and electro-osmotic effects. Hybridization of single-stranded DNA with single-stranded sections of the crosslinks provides a novel means of altering gel mechanical properties. As a step toward exploring this means of altering gel mechanical properties, we report here on a study of the use of electrophoresis to introduce single stranded DNA into DNA crosslinked gels. Changes in elastic properties of the gel, before and after electrophoresis, were measured.

UR - http://www.scopus.com/inward/record.url?scp=1842509198&partnerID=8YFLogxK

U2 - 10.1115/IMECE2003-43446

DO - 10.1115/IMECE2003-43446

M3 - Conference article

AN - SCOPUS:1842509198

SN - 1071-6947

VL - 55

SP - 131

EP - 132

JO - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED

JF - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED

T2 - 2003 ASME International Mechanical Engineering Congress

Y2 - 15 November 2003 through 21 November 2003

ER -

The migration of dna into a DNA-crosslinked gel using electrophoresis

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