Unzipping and shearing DNA with electrophoresed nanoparticles in hydrogels

Keitel Cervantes-Salguero; Ibuki Kawamata; Shin Ichiro M. Nomura; Satoshi Murata

doi:10.1039/c7cp02214j

Unzipping and shearing DNA with electrophoresed nanoparticles in hydrogels

Keitel Cervantes-Salguero
, Ibuki Kawamata
, Shin Ichiro M. Nomura
, Satoshi Murata

Tohoku University

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We show electric control of unzipping and shearing dehybridization of a DNA duplex anchored to a hydrogel. Tensile force is applied by electrophoresing (25 V cm^-1) gold nanoparticles pulling the DNA duplex. The pulled DNA strand is gradually released from the hydrogel. The unzipping release rate is faster than shearing; for example, 3-fold for a 15 base pair duplex, which helps to design electrically driven DNA devices.

Original language	English
Pages (from-to)	13414-13418
Number of pages	5
Journal	Physical Chemistry Chemical Physics
Volume	19
Issue number	21
Early online date	5 May 2017
DOIs	https://doi.org/10.1039/c7cp02214j
State	Published - 7 Jun 2017
Externally published	Yes

Keywords

Acrylic Resins/chemistry
DNA/chemistry
Electrophoresis
Gold/chemistry
Hydrogels/chemistry
Metal Nanoparticles/chemistry
Nucleic Acid Hybridization/drug effects

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10.1039/c7cp02214j

Cite this

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title = "Unzipping and shearing DNA with electrophoresed nanoparticles in hydrogels",

abstract = "We show electric control of unzipping and shearing dehybridization of a DNA duplex anchored to a hydrogel. Tensile force is applied by electrophoresing (25 V cm-1) gold nanoparticles pulling the DNA duplex. The pulled DNA strand is gradually released from the hydrogel. The unzipping release rate is faster than shearing; for example, 3-fold for a 15 base pair duplex, which helps to design electrically driven DNA devices.",

keywords = "Acrylic Resins/chemistry, DNA/chemistry, Electrophoresis, Gold/chemistry, Hydrogels/chemistry, Metal Nanoparticles/chemistry, Nucleic Acid Hybridization/drug effects",

author = "Keitel Cervantes-Salguero and Ibuki Kawamata and Nomura, \{Shin Ichiro M.\} and Satoshi Murata",

note = "Publisher Copyright: {\textcopyright} the Owner Societies 2017.",

year = "2017",

month = jun,

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doi = "10.1039/c7cp02214j",

language = "English",

volume = "19",

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TY - JOUR

T1 - Unzipping and shearing DNA with electrophoresed nanoparticles in hydrogels

AU - Cervantes-Salguero, Keitel

AU - Kawamata, Ibuki

AU - Nomura, Shin Ichiro M.

AU - Murata, Satoshi

N1 - Publisher Copyright: © the Owner Societies 2017.

PY - 2017/6/7

Y1 - 2017/6/7

N2 - We show electric control of unzipping and shearing dehybridization of a DNA duplex anchored to a hydrogel. Tensile force is applied by electrophoresing (25 V cm-1) gold nanoparticles pulling the DNA duplex. The pulled DNA strand is gradually released from the hydrogel. The unzipping release rate is faster than shearing; for example, 3-fold for a 15 base pair duplex, which helps to design electrically driven DNA devices.

AB - We show electric control of unzipping and shearing dehybridization of a DNA duplex anchored to a hydrogel. Tensile force is applied by electrophoresing (25 V cm-1) gold nanoparticles pulling the DNA duplex. The pulled DNA strand is gradually released from the hydrogel. The unzipping release rate is faster than shearing; for example, 3-fold for a 15 base pair duplex, which helps to design electrically driven DNA devices.

KW - Acrylic Resins/chemistry

KW - DNA/chemistry

KW - Electrophoresis

KW - Gold/chemistry

KW - Hydrogels/chemistry

KW - Metal Nanoparticles/chemistry

KW - Nucleic Acid Hybridization/drug effects

UR - https://www.scopus.com/pages/publications/85024091272

U2 - 10.1039/c7cp02214j

DO - 10.1039/c7cp02214j

M3 - Article

C2 - 28513698

AN - SCOPUS:85024091272

SN - 1463-9076

VL - 19

SP - 13414

EP - 13418

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

IS - 21

ER -

Unzipping and shearing DNA with electrophoresed nanoparticles in hydrogels

Abstract

Keywords

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