TY - JOUR
T1 - Useful modifications of the Escherichia coli expression plasmid pJL6
AU - Lautenberger, James A.
AU - Seth, Arun
AU - Jorcyk, Cheryl
AU - Papas, Takis S.
PY - 1984
Y1 - 1984
N2 - Several useful modifications of the Escherichia coli expression plasmid pJL6 have been constructed. These include the introduction of a wide variety of restriction sites by the cloning of oligonucleotide linker DNA or of a short DNA fragment containing many sites. These plasmids can allow the expression of genes adjacent to one of the restriction sites provided. Another plasmid, pJLA16, allows the cloning of blunt-ended DNA adjacent to the fragment of phage gene, thus allowing fusions of this gene fragment with target gene DNA. This facilitates the expression of genes on blunt-ended DNA fragments that are either directly the product of restriction enzyme digestion or fragments that are resected with Bal-31 nuclease.
AB - Several useful modifications of the Escherichia coli expression plasmid pJL6 have been constructed. These include the introduction of a wide variety of restriction sites by the cloning of oligonucleotide linker DNA or of a short DNA fragment containing many sites. These plasmids can allow the expression of genes adjacent to one of the restriction sites provided. Another plasmid, pJLA16, allows the cloning of blunt-ended DNA adjacent to the fragment of phage gene, thus allowing fusions of this gene fragment with target gene DNA. This facilitates the expression of genes on blunt-ended DNA fragments that are either directly the product of restriction enzyme digestion or fragments that are resected with Bal-31 nuclease.
UR - http://www.scopus.com/inward/record.url?scp=0021611862&partnerID=8YFLogxK
U2 - 10.1016/0735-0651(84)90007-4
DO - 10.1016/0735-0651(84)90007-4
M3 - Article
AN - SCOPUS:0021611862
SN - 0735-0651
VL - 1
SP - 63
EP - 66
JO - Gene Analysis Techniques
JF - Gene Analysis Techniques
IS - 4
ER -