TY - JOUR
T1 - Vibrational echo experiments on red blood cells
T2 - Comparison of the dynamics of cytoplasmic and aqueous hemoglobin
AU - McClain, Brian L.
AU - Finkelstein, Ilya J.
AU - Fayer, M. D.
PY - 2004/7/11
Y1 - 2004/7/11
N2 - Ultrafast spectrally resolved stimulated vibrational echo experiments measure the dephasing of the CO stretching mode of hemoglobin-CO (Hb-CO) inside living human erythrocytes (red blood cells). A method is presented to overcome the adverse impact on the vibrational echo signal from the strong light scattering caused by the cells. The results are compared to experiments on Hb-CO aqueous solutions. It is demonstrated that the dynamics of the protein as sensed by the CO ligand are the same inside the erythrocytes and in aqueous solution, but differences in the absorption spectra show that the cell affects the protein's potential energy surface.
AB - Ultrafast spectrally resolved stimulated vibrational echo experiments measure the dephasing of the CO stretching mode of hemoglobin-CO (Hb-CO) inside living human erythrocytes (red blood cells). A method is presented to overcome the adverse impact on the vibrational echo signal from the strong light scattering caused by the cells. The results are compared to experiments on Hb-CO aqueous solutions. It is demonstrated that the dynamics of the protein as sensed by the CO ligand are the same inside the erythrocytes and in aqueous solution, but differences in the absorption spectra show that the cell affects the protein's potential energy surface.
UR - http://www.scopus.com/inward/record.url?scp=3042579574&partnerID=8YFLogxK
U2 - 10.1016/j.cplett.2004.05.080
DO - 10.1016/j.cplett.2004.05.080
M3 - Article
AN - SCOPUS:3042579574
SN - 0009-2614
VL - 392
SP - 324
EP - 329
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 4-6
ER -