Abstract
Formula presented Protein farnesyltransferase (PFTase) catalyzes alkylation of cysteine residues by farnesyl diphosphate (FPP). The dissociation constants for the PFTase-peptide analogue complexes for the series of analogues fl-RTRC(X)VIA (X = H, methyl, dodecyl, farnesyl) were measured by fluorescence anisotropy. The results indicate that an ionizable sulfhydryl moiety is important for substrate binding and the farnesyl group in the product facilitates binding.
Original language | English |
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Pages (from-to) | 815-817 |
Number of pages | 3 |
Journal | Organic Letters |
Volume | 1 |
Issue number | 5 |
DOIs | |
State | Published - 9 Sep 1999 |